Changes in serum citrullinated fibrinogen concentration associated with the phase of bacteremia patients

• Published in: Clinica chimica acta Vol. 512; pp. 127 - 134
• Main Authors: Fujimura, Satoshi, Higuchi, Yumiko, Usami, Yoko, Yamaura, Makoto, Higuchi, Tsukasa, Terasawa, Fumiko, Okumura, Nobuo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2021
• Subjects: Arthritis, Rheumatoid; Bacteremia; Bacteremia - diagnosis; Citrullinated fibrinogen; Citrullinated histone H3; Enzyme-linked immunosorbent assay; Fibrinogen; Humans; Neutrophil extracellular traps; Neutrophils; Peptidyl arginine deiminase; Protein-Arginine Deiminases; Peptidyl arginine deiminase; Citrullinated histone H3; Neutrophil extracellular traps; Citrullinated fibrinogen; Bacteremia; Enzyme-linked immunosorbent assay
• ISSN: 0009-8981; 1873-3492
• DOI: 10.1016/j.cca.2020.10.038

•An ELISA system specific for evaluating C-Fbg levels was established.•Serum C-Fbg levels increased in patients with bacteremia.•Serum C-Fbg levels were positively correlated with WBC and neutrophil counts. Citrullinated fibrinogen (C-Fbg) has been detected in rheumatoid arthritis; however, few studies have reported the role of C-Fbg in other inflammatory diseases. This study aimed to clarify the changes in serum C-Fbg associated with the bacteremia phase. We measured serum C-Fbg concentration in bacteremia patients. C-Fbg levels at each phase of bacteremia, classified by white blood cell (WBC) count and neutrophil left shift change, were compared with those of healthy control (HC). The correlation between C-Fbg concentration and certain inflammatory markers, or citrullinated histone H3 concentration was assessed. Multiple linear regression (MLR) analysis was used to examine the association of log C-Fbg with certain inflammatory markers. Serum C-Fbg levels were significantly higher in bacteremia patients than in HC (p < 0.001) and positively correlated with WBC and neutrophil count. Further, C-Fbg levels were significantly higher in phases III and IV of bacteremia than in HC (p < 0.001). MLR analysis indicated that log C-Fbg had a stronger relationship with log neutrophil counts than other certain inflammatory markers (p < 0.01). Serum C-Fbg levels increased in bacteremia patients, and this was consistent with an influx of neutrophils into the blood stream in accordance with the bacteremia phase.