Spectrophotometric method for the assay of human blood coagulation factor VIII

A spectrophotometric method for the assay of human blood coagulation factor VIII in plasma is presented. The chromogenic assay for factor VIII:C in plasma is performed in 3 steps: activation of factor VIII by thrombin; activation of factor X in a mixture of factor X, factor IXa, phospholipids/Ca2+ a...

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Bibliographic Details
Published inHaemostasis Vol. 17; no. 1-2; p. 14
Main Authors van Dieijen, G, van Dieijen-Visser, M P, Franssen, J, Hemker, H C
Format Journal Article
LanguageEnglish
Published Switzerland 01.01.1987
Subjects
Blood Coagulation Tests
Factor VIII - metabolism
Humans
Reference Values
Spectrophotometry - instrumentation
Spectrophotometry - methods
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Summary:A spectrophotometric method for the assay of human blood coagulation factor VIII in plasma is presented. The chromogenic assay for factor VIII:C in plasma is performed in 3 steps: activation of factor VIII by thrombin; activation of factor X in a mixture of factor X, factor IXa, phospholipids/Ca2+ and plasma containing activated factor VIII, and determination of the rate of factor Xa formation with the chromogenic substrate S2337. Within-assay variation was between 5 and 6.9% for factor VIII:C activities between 20 and 150%. Clotting and chromogenic factor VIII:C activities were compared in plasma of 50 normal healthy donors (coefficient of correlation r = 0.83).
ISSN:0301-0147
DOI:10.1159/000215554