The long non-coding RNA ZFAS1 promotes colorectal cancer progression via miR200b/ZEB1 axis

• Published in: Pathology, research and practice Vol. 247; p. 154567
• Main Authors: Salman, Islam T., Abulsoud, Ahmed I., Abo-Elmatty, Dina M., Fawzy, Amal, Mesbah, Noha M., Saleh, Samy M.
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.07.2023
• Subjects: Colorectal cancer; LncRNA; MiR200b; ZEB1; ZFASI; CRP; lncRNAs; MiR200b; RT; ZFAS1; Colorectal cancer; LncRNA; ALT; SD; miRNA; cDNA; CEA; AST; qRT-PCR; ROC; IQR; EMT; KLF2; r; PC; ZEB1; ZNFX1; CRC; CA 19–9; Sn; MET; Sp; ZFASI
• ISSN: 0344-0338; 1618-0631; 1618-0631
• DOI: 10.1016/j.prp.2023.154567

Colorectal cancer (CRC) is a common tumor worldwide. CRC is influenced by several types of miRNAs and long non-coding RNAs. This study aims to evaluate the correlation of lncRNA ZFAS1/ miR200b/ ZEB1 protein with presence of CRC. Quantitative real-time polymerase chain reaction was used to measure serum expression of lncRNA ZFAS1 and microRNA-200b in 60 CRC patients and 28 control subjects. ZEB1 protein in serum was measured by ELISA. Lnc ZFAS1 and ZEB1 were up-regulated in CRC patients in compare to control subjects while miR-200b was down-regulated. There was a linear correlation between ZAFS1 expression and miR-200b and ZEB1 in CRC. ZFAS1 is a key player of CRC progression and could be a potential therapeutic target by sponging miR-200b. In-addition the association between ZFAS1, miR-200b and ZEB1 highlights their potential value as a novel diagnostic biomarker in human CRC. [Display omitted]