Shark centra microanatomy and mineral density variation studied with laboratory microComputed Tomography

• Published in: Journal of structural biology Vol. 214; no. 1; p. 107831
• Main Authors: Morse, Paul E., Stock, Michala K., James, Kelsey C., Natanson, Lisa J., Stock, Stuart R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2022
• Subjects: Carcharhiniformes; Centra; Lamniformes; microComputed Tomography; Shark; Vertebra; Centra; Vertebra; microComputed Tomography; Carcharhiniformes; Lamniformes; Shark
• ISSN: 1047-8477; 1095-8657
• DOI: 10.1016/j.jsb.2022.107831

[Display omitted] •Centra of shark vertebrae from three species of Lamniformes (Alopias vulpinus, Carcharodon carcharias and Isurus oxyrinchus) and three species of Carcharhiniformes (Carcharhinus plumbeus, Carcharhinus obscurus and Prionace glauca) were imaged with laboratory microComputed Tomography (microCT) using volume element (voxel) sizes between 16 and 24 µm.•Linear attenuation coefficients were the same in the hourglass double-cone (corpus calcarea) and lamellar intermedialia of the lamniforms but were smaller in the wedge-shaped intermedialia than in the hourglass of the carcharhiniforms.•All centra contained growth bands visible as small changes in linear attenuation coefficient.•The cross-sections of the cones were close to circular.•Cartilage canals were prominent structure in the intermedialia of all species.•Somewhat higher calcification levels were seen around the periphery of cartilage canals and of outer surfaces of the intermedialia and hourglass. Centra of shark vertebrae from three species of Lamniformes (Alopias vulpinus, Carcharodon carcharias and Isurus oxyrinchus) and three species of Carcharhiniformes (Carcharhinus plumbeus, Carcharhinus obscurus and Prionace glauca) were imaged with laboratory microcomputed Tomography (microCT) using volume element (voxel) sizes between 16 and 24 µm. Linear attenuation coefficients were the same in the corpus calcarea (hour-glass-shaped cone) and intermedialia of the lamniforms but were smaller in the intermedialia than in the corpus calcarea of the carcharhiniforms. All centra contained growth bands which were visible as small changes in linear attenuation coefficient. In all six cases, the cross-sections of the cones were close to circular, and the cone angles matched those reported in the literature. Cartilage canals were a prominent structure in the intermedialia of all species, 3D renderings of centra of C. obscurus and I. oxyrinchus diameters showed these canals ran radially outward from the cone walls, and canal diameters were consistent with the limited numerical values in the literature. Somewhat higher calcification levels around the periphery of cartilage canals and of outer surfaces of the intermedialia and corpus calcerea suggest microstructural variation exists at scale below that which can be resolved in the present data sets.