Inhibitory actions of lupinifolin isolated from Derris reticulata stem against carbohydrate-digesting enzymes

• Published in: Pharmacognosy research Vol. 12; no. 2; pp. 102 - 106
• Main Authors: Pulbutr, Pawitra, Nantana, Pichnatcha, Suksabai, Surabot, Mudjupa, Chawannuch, Denchai, Rattazart, Rattanakiat, Sakulrat, Dhammaraj, Taweesak
• Format: Journal Article
• Language: English
• Published: Bangalore Wolters Kluwer India Pvt. Ltd 01.04.2020; Medknow Publications and Media Pvt. Ltd; Phcog.net
• Subjects: Amylases; Carbohydrates; Diabetes; Diabetes mellitus; Diabetes therapy; Diagnostic imaging; Enzyme kinetics; Glucose; Herbal medicine; Hyperglycemia; Mass spectrometry; Medical research; Medicinal plants; NMR; Nuclear magnetic resonance; Software; Spectroscopy; Statistical analysis; Type 2 diabetes; Thailand; Germany; lupinifolin; α-glucosidase; diabetes mellitus; α-amylase; Derris reticulate
• ISSN: 0974-8490; 0976-4836; 0974-8490
• DOI: 10.4103/pr.pr_117_19

Background: Postprandial hyperglycemia is linked with the development of diabetic complications. Inhibition against the carbohydrate-digesting enzymes, specifically pancreatic α-amylase and intestinal α-glucosidase, delays carbohydrate digestion and subsides postprandial plasma glucose (PPG) levels. Objectives: This study aimed to examine the effects of lupinifolin, purified from Derris reticulata stem, a medicinal plant traditionally used for the treatment of diabetes mellitus, on in vitro α-amylase and α-glucosidase enzyme activities and enzyme kinetics. Subjects and Methods: The identification of the isolated phytochemical was performed using nuclear magnetic resonance-mass spectrometry (NMR-MS) spectrometry.In vitro pancreatic α-amylase and α-glucosidase activities and enzyme kinetics were determined using the enzymatic colorimetric methods. Results: The purified phytochemical was identified as lupinifolin from the NMR-MS spectrometry. The isolated lupinifolin produced a significant α-amylase and α-glucosidase inhibitory actions with the median inhibitory concentrations of 3.43 ± 0.77 and 56.29 ± 11.64 μg/mL, respectively. The study of enzyme kinetics showed that lupinifolin exhibited the mode of non-competitive and mixed enzyme inhibitions against α-amylase and α-glucosidase, respectively. Conclusion: The potent α-amylase and α-glucosidase inhibitory actions of lupinifolin derived from D. reticulata stem evidently suggest its potential use as an alternative for the control of PPG.