Comprehensive transcriptome and proteome analyses reveal a novel sodium chloride responsive gene network in maize seed tissues during germination

• Published in: Plant, cell and environment Vol. 44; no. 1; pp. 88 - 101
• Main Authors: Chen, Mo‐Xian, Lu, Chong‐Chong, Sun, Peng‐Cheng, Nie, Yong‐Xin, Tian, Yuan, Hu, Qi‐Juan, Das, Debatosh, Hou, Xuan‐Xuan, Gao, Bei, Chen, Xi, Liu, Shou‐Xu, Zheng, Cheng‐Chao, Zhao, Xiang‐Yu, Dai, Lei, Zhang, Jianhua, Liu, Ying‐Gao
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.01.2021; Wiley Subscription Services, Inc
• Subjects: Abscisic acid; Alternative splicing; Corn; Embryos; Endosperm; Environmental factors; Gene expression; Gene sequencing; Germination; Proteomes; Proteomics; quantitative proteomics; Regulatory mechanisms (biology); Ribonucleic acid; RNA; Salts; Seed germination; Seedlings; Seeds; Sodium chloride; Splicing; splicing factor; SWATH‐MS; Transcription; transcriptome; Water control; Zea mays; Zea mays; transcriptome; SWATH-MS; seed germination; quantitative proteomics; sodium chloride; splicing factor
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/pce.13849

Germination is a plant developmental process by which radicle of mature seeds start to penetrate surrounding barriers for seedling establishment and multiple environmental factors have been shown to affect it. Little is known how high salinity affects seed germination of C4 plant, Zea mays. Preliminary germination assay suggested that isolated embryo alone was able to germinate under 200 mM NaCl treatment, whereas the intact seeds were highly repressed. We hypothesized that maize endosperm may function in perception and transduction of salt signal to surrounding tissues such as embryo, showing a completely different response to that in Arabidopsis. Since salt response involves ABA, we analysed in vivo ABA distribution and quantity and the result demonstrated that ABA level in isolated embryo under NaCl treatment failed to increase in comparison with the water control, suggesting that the elevation of ABA level is an endosperm dependent process. Subsequently, by using advanced profiling techniques such as RNA sequencing and SWATH‐MS‐based quantitative proteomics, we found substantial differences in post‐transcriptional and translational changes between salt‐treated embryo and endosperm. In summary, our results indicate that these regulatory mechanisms, such as alternative splicing, are likely to mediate early responses to salt stress during maize seed germination. This study demonstrated that maize endosperm may function in sensation and transduction of salt signal to surrounding tissues during salt stress, which is a completely different responsive model from that of Arabidopsis.