Characterization of lipopeptide biosurfactant produced by a carbazole‐degrading bacterium Roseomonas cervicalis: The role of biosurfactant in carbazole solubilisation

• Published in: Journal of applied microbiology Vol. 132; no. 2; pp. 1062 - 1078
• Main Authors: Mukherjee, Ashis K., Chanda, Abhishek, Mukherjee, Indrajit, Kumar, Pawan
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.02.2022
• Subjects: Bacteria; Biodegradation; Biodegradation, Environmental; Bioremediation; biosurfactant; Carbazole; Carbazoles; Crude oil; Genomes; Glucose; High-performance liquid chromatography; Hydrocarbon-degrading bacteria; Intracellular; Lipopeptides; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Methylobacteriaceae; NMR; Nuclear magnetic resonance; Oil pollution; PAH biodegradation; Petroleum; Petroleum - analysis; Petroleum hydrocarbons; Polycyclic aromatic hydrocarbons; Proteins; proteomic analysis; Proteomics; Roseomonas cervicalis; Sediment pollution; Soil contamination; Soil pollution; Solubilization; Surface-Active Agents; Surfactants; Tandem Mass Spectrometry; Roseomonas cervicalis; carbazole; PAH biodegradation; biosurfactant; proteomic analysis
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/jam.15258

Aim Characterization of biosurfactant produced by a carbazole‐degrading bacterium Roseomonas cervicalis and proteomic analysis of intracellular proteins of bacterium while growing on glucose and carbazole medium. Methods and Results The bacterium R. cervicalis was isolated from a soil sample contaminated with crude petroleum oil. PCR amplification ascertained the existence of some hydrocarbon‐degrading catabolic genes (alkB and PAH‐RHDα, C12O, and C23O) in the bacterial genome. GC‐MS and RP‐HPLC analyses demonstrated 62% and 60% carbazole degradation, respectively, by R. cervicalis 144 h post‐incubation at 37℃ and pH 6.5. Due to the paucity of protein databases, expressions of only 29 and 14 intracellular proteins were explicitly recognized and quantitated by mass spectrometry analysis when R. cervicalis was grown in carbazole and glucose medium, respectively. FTIR, NMR and HR‐MS/MS analyses demonstrated the lipopeptide nature of the purified biosurfactant produced by R. cervicalis. The biosurfactant is also presumed to assist in the solubilization of carbazole. Conclusion The isolated R. cervicalis strain is a potential candidate for the bioremediation of carbazole in petroleum‐oil‐contaminated sites. Significance and Impact of the Study This is the first report of the promising R. cervicalis strain proficient in carbazole biodegradation.