Metabolomic differences of exhaled breath condensate among children with and without asthma
Background There remains an unmet need in objective tests for diagnosing asthma in children. The objective of this study was to investigate the potential of metabolomic profiles of exhaled breath condensate (EBC) to discriminate stable asthma in Asian children in the community. Methods One hundred a...
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Published in | Pediatric allergy and immunology Vol. 32; no. 2; pp. 264 - 272 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Wiley Subscription Services, Inc
01.02.2021
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Subjects | |
Online Access | Get full text |
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Summary: | Background
There remains an unmet need in objective tests for diagnosing asthma in children. The objective of this study was to investigate the potential of metabolomic profiles of exhaled breath condensate (EBC) to discriminate stable asthma in Asian children in the community.
Methods
One hundred and sixty‐five Asian children (92 stable asthma and 73 non‐asthmatic controls) participating in a population‐based cohort were enrolled and divided into training and validation sets. Nuclear magnetic resonance‐based metabolomic profiles of EBC samples were analyzed by using orthogonal partial least squares discriminant analysis.
Results
EBC metabolomic signature (lactate, formate, butyrate, and isobutyrate) had an area under the receiver operator characteristic curve (AUC) of 0.826 in discriminating children with and without asthma in the training set, which significantly outperformed FeNO (AUC = 0.574; P < .001) and FEV1/FVC % predicted (AUC = 0.569; P < .001). The AUC for EBC metabolomic signature was 0.745 in the validation set, which was slightly but not significantly lower than in the testing set (P = .282). We further extrapolated two potentially involved metabolic pathways, including pyruvate (P = 1.67 × 10−3; impact: 0.14) and methane (P = 1.89 × 10−3; impact: 0.15), as the most likely divergent metabolisms between children with and without asthma.
Conclusion
This study provided evidence supporting the role of EBC metabolomic signature to discriminate stable asthma in Asian children in the community, with a discriminative property outperforming conventional clinical tests such as FeNO or spirometry. |
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Bibliography: | Funding information This study was supported by the Ministry of Science and Technology of Taiwan (PIs: Yao, MOST 106‐2314‐B‐182‐051‐MY3 and MOST 109‐2314‐B‐182‐042‐MY3; Tsai, MOST 107‐2314‐B‐400‐031‐MY3) or Chang Gung Medical Foundation (CMRPG3F1711 ~ 3F1713, CMRPG3E1201 ~ 3E1205, CMRPG3F0361, and CMRPG3J0121). ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0905-6157 1399-3038 |
DOI: | 10.1111/pai.13368 |