Morphology and Morphogenesis of a Novel Saline Soil Hypotrichous Ciliate, Gonostomum sinicum nov. spec. (Ciliophora, Hypotrichia, Gonostomatidae), Including a Report on the Small Subunit rDNA Sequence

• Published in: The Journal of eukaryotic microbiology Vol. 64; no. 5; pp. 632 - 646
• Main Authors: Lu, Xiaoteng, Huang, Jie, Shao, Chen, Al‐Farraj, Saleh A., Gao, Shan
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.09.2017
• Subjects: Animal morphology; Cell Size; Cirri; Congeners; Cortex; DNA; DNA, Protozoan - genetics; DNA, Ribosomal - genetics; Hypotrichida - genetics; Hypotrichida - growth & development; Hypotrichida - ultrastructure; Longfeng Wetland; Morphogenesis; morphogeny; Morphology; New species; ontogenesis; Phylogeny; Primordia; Saline soils; Saline Waters; Sequence Analysis, DNA - methods; Sequencing; Soil; Soil - parasitology; terrestrial habitat; new species; ontogenesis; Longfeng Wetland; morphogeny; terrestrial habitat
• ISSN: 1066-5234; 1550-7408
• DOI: 10.1111/jeu.12398

Morphology, cirral pattern, and morphogenesis of the new saline soil hypotrich, Gonostomum sinicum nov. spec. collected from Longfeng Wetland in Daqing, north China, were studied, using detailed live observations and protargol‐stained specimens. The new species is characterized as follows: (i) a size in vivo of 100–125 × 30–40 μm, (ii) colorless cortical granules, 0.5 μm across, arranged in short rows, (iii) an adoral zone composed of 28–33 membranelles, (iv) three or four frontoventral rows, one of which extends onto the postoral area, (v) left and right marginal rows composed of 18–27 and 21–35, cirri, respectively, and (vi) usually two transverse cirri. Morphogenesis is as usual for the genus Gonostomum, i.e. the cirral primordia II–VI are primary primordia which split into two sets for proter and opisthe in division middle stages, except for anlage I which develops independently. However, the number of frontoventral transverse anlagen is either five or six not only in different individuals but even in proter and opisthe of the same divider. The phylogenetic analyses based on SSU rDNA sequences showed that the genus Gonostomum is nonmonophyletic, indicating that the patterns of cirri and dorsal kineties are homoplasious characters. The new species G. sinicum nov. spec. is perhaps closely related to Cotterillia bromelicola and two congeners.