Confocal laser scanning microscopy evaluation of roots subjected to activation protocol in endodontic microsurgery

• Published in: Australian endodontic journal Vol. 48; no. 1; pp. 77 - 81
• Main Authors: Iandolo, Alfredo, Abdellatif, Dina, Barbosa, Ana Flávia A., Scelza, Giuseppe, Gasparro, Roberta, Sammartino, Pasquale, Silva, Emmanuel J. N. L.
• Format: Journal Article
• Language: English
• Published: Australia Wiley Subscription Services, Inc 01.04.2022
• Subjects: Confocal microscopy; Dental Pulp Cavity; Dentin; dentinal tubules penetration; endodontic microsurgery; Endodontics; irrigant penetration; Microscopes; Microscopy; Microscopy, Confocal; Microsurgery; Premolars; retro‐preparation; Rhodamine; Root Canal Irrigants - therapeutic use; Root Canal Preparation - methods; Sodium Hypochlorite; ultrasonic; endodontic microsurgery; dentinal tubules penetration; retro-preparation; ultrasonic; irrigant penetration
• ISSN: 1329-1947; 1747-4477; 1747-4477
• DOI: 10.1111/aej.12598

This study evaluated the penetration of the irrigant subject to activation after performing retro‐preparation in endodontic microsurgery. Forty mandibular premolars were prepared and filled. Subsequently, 1 mm from the root apex was cut using a multi‐blade bur and the retro‐preparation was performed. In group 1, the retro‐cavity was cleaned with 2 mL of saline and then with 2 mL of 5.25% NaOCl gel mixed with 0.1% Rhodamine B. In group 2, the retro‐cavity was cleaned with 2 mL of saline, 17% EDTA gel was activated ultrasonically for 30 s and 5.25% NaOCl gel was mixed with 0.1% Rhodamine B and activated for 30 s. After 7 days, the roots were cut at 1mm from the apex and the slices were evaluated with confocal laser scanning microscopy. The samples of group 2 showed statistically better results. The activation protocol proposed resulted in enhanced irrigant penetration when compared to the traditional.