Imaging innate immunity
Innate immunity is the first line of defense against infectious intruders and also plays a major role in the development of sterile inflammation. Direct microscopic imaging of the involved immune cells, especially neutrophil granulocytes, monocytes, and macrophages, has been performed since more tha...
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Published in | Immunological reviews Vol. 306; no. 1; pp. 293 - 303 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Wiley Subscription Services, Inc
01.03.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Innate immunity is the first line of defense against infectious intruders and also plays a major role in the development of sterile inflammation. Direct microscopic imaging of the involved immune cells, especially neutrophil granulocytes, monocytes, and macrophages, has been performed since more than 150 years, and we still obtain novel insights on a frequent basis. Initially, intravital microscopy was limited to small‐sized animal species, which were often invertebrates. In this review, we will discuss recent results on the biology of neutrophils and macrophages that have been obtained using confocal and two‐photon microscopy of individual cells or subcellular structures as well as light‐sheet microscopy of entire organs. This includes the role of these cells in infection defense and sterile inflammation in mammalian disease models relevant for human patients. We discuss their protective but also disease‐enhancing activities during tumor growth and ischemia‐reperfusion damage of the heart and brain. Finally, we provide two visions, one experimental and one applied, how our knowledge on the function of innate immune cells might be further enhanced and also be used in novel ways for disease diagnostics in the future. |
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Bibliography: | This article is part of a series of reviews covering Insights into immune function from imaging appearing in Volume 306 of Immunological Reviews. Funding information We acknowledge funding by the Ministry of Culture and Science of North Rhine‐Westphalia, the Governing Mayor of Berlin including Science and Research, and the Federal Ministry of Education and Research to MG and AG MG was supported by the Deutsche Forschungsgemeinschaft [GU 769/13‐1 (KFO 337); INST 20876/323‐1, GU 769/10‐1] and the Bundesministerium für Bildung und Forschung (BMBF, MSCoreSys JRG AMBIOM, 031L0272) ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 |
ISSN: | 0105-2896 1600-065X |
DOI: | 10.1111/imr.13048 |