A pepper RING‐finger E3 ligase, CaFIRF1, negatively regulates the high‐salt stress response by modulating the stability of CaFAF1

• Published in: Plant, cell and environment Vol. 47; no. 4; pp. 1319 - 1333
• Main Authors: Bae, Yeongil, Baek, Woonhee, Lim, Chae Woo, Lee, Sung Chul
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.04.2024
• Subjects: ABA; Abiotic stress; Biodegradation; Capsicum annuum; Degradation; Peppers; Phenotypes; Proteasome 26S; Proteasomes; protein degradation; Proteins; RING finger proteins; Salinity tolerance; Salt tolerance; Ubiquitin-protein ligase; Ubiquitination; ubiquitin‐proteasome system; Vegetables; ABA; ubiquitin-proteasome system; ubiquitination; protein degradation
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/pce.14818

Controlling protein stability or degradation via the ubiquitin‐26S proteasome system is a crucial mechanism in plant cellular responses to stress conditions. Previous studies have revealed that the pepper FANTASTIC FOUR‐like gene, CaFAF1, plays a positive role in salt tolerance and that, in this process, CaFAF1 protein degradation is delayed. Here, we sought to isolate the E3 ligases potentially responsible for modulating CaFAF1 protein stability in response to salt stress. The pepper RING‐type E3 ligase CaFIRF1 (Capsicum  annuum  FAF1  Interacting  RING  Finger protein  1) was found to interact with and ubiquitinate CaFAF1, leading to the degradation of CaFAF1 proteins. In response to high‐salt treatments, CaFIRF1‐silenced pepper plants exhibited tolerant phenotypes. In contrast, co‐silencing of CaFAF1 and CaFIRF1 led to increased sensitivity to high‐salt treatments, revealing that CaFIRF1 functions upstream of CaFAF1. A cell‐free degradation analysis showed that high‐salt treatment suppressed CaFAF1 protein degradation via the 26S proteasome pathway, in which CaFIRF1 is functionally involved. In addition, an in vivo ubiquitination assay revealed that CaFIRF1‐mediated ubiquitination of CaFAF1 proteins was reduced by high‐salt treatment. Taken together, these findings suggest that the degradation of CaFAF1 mediated by CaFIRF1 has a critical role in pepper plant responses to high salinity. Summary statement We previously reported that pepper FANTASTIC FOUR‐like protein CaFAF1 functions as a positive modulator of high salt response. Building on this in the present study, we identified the RING type E3 ligase CaFIRF1 as an interactor of CaFAF1, which plays a negative role in response to high salt stress via regulation of CaFAF1 stability.