Association of single nucleotide autophagy‐related protein 5 gene polymorphism rs2245214 with susceptibility to non–small cell lung cancer

• Published in: Journal of cellular biochemistry Vol. 120; no. 2; pp. 1924 - 1931
• Main Authors: Nikseresht, Mohsen, Shahverdi, Maryam, Dehghani, Mehdi, Abidi, Hassan, Mahmoudi, Reza, Ghalamfarsa, Ghasem, Manzouri, Leila, Ghavami, Saeid
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.02.2019
• Subjects: Alleles; Apoptosis; ATG5; Autophagy; Cancer; Cytosine; Females; Gene expression; Gene polymorphism; Gene regulation; Genes; Genotype & phenotype; Guanine; Lung cancer; Lungs; Males; Non-small cell lung carcinoma; non–small cell lung cancer (NSCLC); Patients; Phagocytosis; Polymorphism; Proteins; Regression analysis; Restriction fragment length polymorphism; Risk reduction; polymorphism; autophagy; non-small cell lung cancer (NSCLC); ATG5
• ISSN: 0730-2312; 1097-4644; 1097-4644
• DOI: 10.1002/jcb.27467

Introduction: Autophagy is a mechanism that is involved in the regulation of cellular life, apoptosis, and stemness while its intervening genes play important functions in various cancers including lung cancer. ATG5 is one of the key genes for the regulation of the autophagy pathway. In this study, our team has investigated the potential relationship between ATG5 gene polymorphism rs2245214 with non–small cell lung cancer (NSCLC) in a subpopulation of patients from southern Iran. In this study, 34 patients with NSCLC (20 males and 14 females [mean age: 12.86 ± 60.47 years]) and 50 healthy subjects (30 males and 20 females [mean age: 13.09 ± 56.62 years]) were studied in terms of the genotype of the ATG5 gene. We used restriction fragment length polymorphism and analyzed the results using SPSS software (v.23). The results revealed that subjects harboring the guanine/cytosine (GC) genotype of the rs2245214 ATG5 gene polymorphism had suffered less from NSCLC, whereas the prevalence of the C‐allele of this polymorphism was significantly higher in patients with NSCLC ( P < 0.05). On the basis of the results of logistic regression, the presence of this C‐allele may predict the risk of lung cancer ( P value = 0.011; OR, 3.52; 95% CI, 1.33‐9.26). This study concludes that the C‐allele of the rs2245214 ATG5 gene polymorphism is associated with increased susceptibility to NSCLC, whereas the GC genotype of this polymorphism is associated with decreased risk and might therefore have a protective role in the development of NSCLC.