Determination of oxytetracycline by flow injection with chemiluminescence detection

• Published in: Luminescence (Chichester, England) Vol. 20; no. 3; pp. 129 - 134
• Main Authors: Guo, Liangqia, Xie, Zenghong, Bian, Xintong, Lin, Xucong, Zhang, Weilin, Liu, Xiaohua, Chen, and Guonan
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.05.2005
• Subjects: 2,2'-Dipyridyl - analogs & derivatives; 2′-bipyridine; Calibration; chemiluminescence; flow injection analysis; Luminescent Measurements - methods; Luminescent Measurements - standards; Oxidation-Reduction; oxytetracycline; Oxytetracycline - analysis; Oxytetracycline - standards; Pharmaceutical Preparations - analysis; ruthenium (II); Sulfuric Acids; tris; tris(2,2′‐bipyridine)
• ISSN: 1522-7235; 1522-7243
• DOI: 10.1002/bio.827

A flow‐injection chemiluminescent method for the determination of oxytetracycline was developed. The method is based on an enhancement by oxytetracycline of the chemiluminescence light emission of tris(2,2′‐bipyridine) ruthenium (II), generated by the continuous oxidation of tris(2,2′‐bipyridine) ruthenium (II) by cerium (IV) sulphate in sulphuric acid. Under the optimum conditions, the calibration curve was linear over the range 1.0 × 10−7–1.0 × 10−5 g[sol ]mL for oxytetracycline with the linear equation: ΔINT = 148.77 × C + 0.6637 (R2 = 0.9994). The detection limit was 4.52 × 10−8 g[sol ]mL. The proposed method was also successfully used to determine oxytetracycline in pharmaceutical formulations. The mean recovery of determination of oxytetracycline was 92.73%. A mechanism for the chemiluminescence enhancement by oxytetracycline of tris(2,2′‐bipyridine)–ruthenium (II) and cerium (IV) sulphate system is also proposed. Copyright © 2005 John Wiley & Sons, Ltd.