Determination of oxytetracycline by flow injection with chemiluminescence detection
A flow‐injection chemiluminescent method for the determination of oxytetracycline was developed. The method is based on an enhancement by oxytetracycline of the chemiluminescence light emission of tris(2,2′‐bipyridine) ruthenium (II), generated by the continuous oxidation of tris(2,2′‐bipyridine) ru...
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Published in | Luminescence (Chichester, England) Vol. 20; no. 3; pp. 129 - 134 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
01.05.2005
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Subjects | |
Online Access | Get full text |
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Summary: | A flow‐injection chemiluminescent method for the determination of oxytetracycline was developed. The method is based on an enhancement by oxytetracycline of the chemiluminescence light emission of tris(2,2′‐bipyridine) ruthenium (II), generated by the continuous oxidation of tris(2,2′‐bipyridine) ruthenium (II) by cerium (IV) sulphate in sulphuric acid. Under the optimum conditions, the calibration curve was linear over the range 1.0 × 10−7–1.0 × 10−5 g[sol ]mL for oxytetracycline with the linear equation: ΔINT = 148.77 × C + 0.6637 (R2 = 0.9994). The detection limit was 4.52 × 10−8 g[sol ]mL. The proposed method was also successfully used to determine oxytetracycline in pharmaceutical formulations. The mean recovery of determination of oxytetracycline was 92.73%. A mechanism for the chemiluminescence enhancement by oxytetracycline of tris(2,2′‐bipyridine)–ruthenium (II) and cerium (IV) sulphate system is also proposed. Copyright © 2005 John Wiley & Sons, Ltd. |
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Bibliography: | Ministry of Science and Technology, People's Republic of China ark:/67375/WNG-CDX5WLHT-1 ArticleID:BIO827 2001BA804A26. istex:654699876E84507DAADA081273042C53C98AE9C6 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1522-7235 1522-7243 |
DOI: | 10.1002/bio.827 |