Study on mechanism about long noncoding RNA MALAT1 affecting pancreatic cancer by regulating Hippo‐YAP signaling

• Published in: Journal of cellular physiology Vol. 233; no. 8; pp. 5805 - 5814
• Main Authors: Zhou, Yongping, Shan, Ting, Ding, Wenzhou, Hua, Zhiyuan, Shen, Yijun, Lu, Zhihua, Chen, Bo, Dai, Tu
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.08.2018
• Subjects: Adaptor Proteins, Signal Transducing - metabolism; Apoptosis; Apoptosis - genetics; Assaying; Cancer; Cell Line, Tumor; Cell migration; Cell Movement - genetics; Cell proliferation; Cell Proliferation - genetics; Cholecystokinin; Cytometry; Flow cytometry; Gene Expression - genetics; Hippo‐YAP signaling; Humans; Immunohistochemistry; lncRNA MALAT1; Neoplasm Invasiveness - genetics; Pancreatic cancer; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - pathology; Phosphoproteins - metabolism; Prognosis; Protein-Serine-Threonine Kinases - metabolism; Ribonucleic acid; RNA; RNA Interference; RNA, Long Noncoding - genetics; RNA, Small Interfering - genetics; Signal Transduction; Signaling; Tissues; Transcription Factors; Wound healing; Yes-associated protein; Hippo-YAP signaling; pancreatic cancer; lncRNA MALAT1
• ISSN: 0021-9541; 1097-4652
• DOI: 10.1002/jcp.26357

By investigating the migration and invasion ability in pancreatic cancer, this study probed into the lncRNA MALAT1 molecular mechanism on Hippo‐YAP signaling. The expression of lncRNA MALAT1 in PC tissues and cells was detected by qRT‐PCR and Western blot. The effect of si‐MALAT1 on proliferation was determined by CCK‐8 assay. Cell apoptosis, migration, and invasion were respectively detected by flow cytometry assay, wound healing assay, and transwell assay. Western blot and immunohistochemistry were successively used for detecting LATS1 and YAP1 expression in pancreatic cancer tissues. The microarray analysis determined that lncRNA MALAT1 in pancreatic cancer was highly expressed. LncRNA MALAT1 presented an extremely high expression level in pancreatic cancer tissues and cells. After transfected with si‐MALAT1, the proliferation of AsPC‐1 cells decreased, induce apoptosis of AsPC‐1 cells, and migration and invasion ability were reduced. The tendency of LATS1 expression level was down‐regulated and YAP1 show the opposite trend in the Hippo‐YAP signaling. The in vivo assay was found that the tumor to be small in size and volume, and the expression of Ki‐67 was decreased. High expression of lncRNA MALAT1 in PC disorder the proliferation, apoptosis, and migration and invasion ability via influence Hippo‐YAP signaling pathway. By investigating the migration and invasion ability in pancreatic cancer, this study probed into the lncRNA MALAT1 molecular mechanism on Hippo‐YAP signaling. High expression of lncRNA MALAT1 in PC disorder the proliferation, apoptosis, and migration and invasion ability via influence Hippo‐YAP signaling pathway.