Comparative Transcriptome Analysis Reveals Candidate Genes Related to Structural and Storage Carbohydrate Biosynthesis in Kelp Saccharina japonica (Laminariales, Phaeophyceae)

• Published in: Journal of phycology Vol. 56; no. 5; pp. 1168 - 1183
• Main Authors: Zhang, Linan, Cao, Zengmei, Liang, Guangjin, Li, Xiaojie, Wu, Hao, Yang, Guanpin, Coleman, M.
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.10.2020
• Subjects: alginate; Alginates; Alginic acid; Biofuels; Biosynthesis; Breeding; carbohydrate; Carbohydrate metabolism; Carbohydrate Metabolism - genetics; Carbohydrates; Cell walls; Cellulose; China; Cytoplasm; Encyclopaedias; Encyclopedias; Food industry; Fucoidan; Gene expression; Gene Expression Profiling; Genes; Genomes; Genomics; Identification; Industry; Kelp; Kelp - genetics; Laminarin; Mannitol; Metabolism; Phaeophyceae - genetics; Pharmaceutical industry; Photosynthesis; Raw materials; Saccharina japonica; Seaweed meal; Seaweeds; Sporophytes; Storage; Transcriptome; Trehalose; China; Saccharina japonica; alginate; mannitol; transcriptome; carbohydrate
• ISSN: 0022-3646; 1529-8817
• DOI: 10.1111/jpy.13016

Saccharina japonica is a brown macroalga that has been commercially cultivated in China for almost a century. As a natural raw material, it is widely used in the food and pharmaceutical industries, and it may potentially be useful for biofuel production. However, little is known about the genes involved in carbohydrate biosynthesis, and their regulation is less understood. In this study, the analysis of growth traits and alginate and mannitol contents suggested that sporophyte development could be divided into four stages. Accordingly, we performed transcriptome analysis of the S. japonica sporophyte. In total, 589 million clean reads were generated, and 4,514 novel genes were identified. Gene expression analysis revealed that 2,542 genes were differentially expressed. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that these genes were significantly enriched in “Carbon metabolism,” “Photosynthesis,” and “Photosynthesis‐antenna proteins” pathways, which are important for metabolism of various carbohydrates during sporophyte development. Systematic analysis identified the genes encoding enzymes for the biosynthesis of cell wall carbohydrates (including alginate, fucoidan, and cellulose) and cytoplasm storage carbohydrates (mannitol, laminarin, and trehalose). Among them, some key genes associated with carbohydrate content were further identified based on detailed expression profiling, representing good candidates for further functional studies. This study provides a global view of the carbohydrate metabolism process and an important resource for functional genomics studies in S. japonica. The results obtained lay the basis for elucidating the molecular mechanism of carbohydrate biosynthesis and for genetic breeding of carbohydrates‐related traits in kelp.