Gingival tissue samples from periodontitis patients demonstrate epithelial–mesenchymal transition phenotype

• Published in: Journal of periodontal research Vol. 58; no. 2; pp. 247 - 255
• Main Authors: Saliem, Saif S., Bede, Salwan Y., Abdulkareem, Ali A., Abdullah, Bashar H., Milward, Michael R., Cooper, Paul R.
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.04.2023
• Subjects: beta Catenin - metabolism; Cadherins; Catenin; cytokines; Epithelial-Mesenchymal Transition - genetics; epithelial–mesenchymal transition; Epithelium; Genotype & phenotype; Gingiva; gram‐negative bacteria; Gum disease; Humans; Periodontitis; Periodontium; Phenotype; Phenotypes; Signal transduction; Vimentin; Vimentin - metabolism; gram-negative bacteria; cytokines; epithelial-mesenchymal transition; periodontitis
• ISSN: 0022-3484; 1600-0765; 1600-0765
• DOI: 10.1111/jre.13086

Objective To determine the expression of key epithelial–mesenchymal transition (EMT) markers in gingival tissue samples collected from patients with periodontitis. Background Epithelial–mesenchymal transition is a process responsible for shifting epithelial‐phenotype to mesenchymal‐phenotype leading to loss of epithelial‐barrier function. Thus, EMT could be involved as a pathogenic mechanism in periodontitis as both conditions share common promoters and signalling pathways. Materials and Methods Gingival tissue samples were collected from patients with periodontitis (case) and healthy periodontium (control). Periodontal parameters including bleeding on probing, probing pocket depth (PPD), and clinical attachment loss were recorded. Paraffinized tissue samples were processed and immunohistochemically stained to determine the expression of key EMT markers which included E‐cadherin, β‐catenin, Snail1 and vimentin. Results The majority of cases (n = 65, 72.2%) were diagnosed with periodontitis stage 3 or 4, grade b or c vs 25 (27.8%) subjects with intact healthy periodontium. Discontinuity of epithelium was detected in up to 80.9% of periodontitis cases associated with reduced number of epithelial layers as compared to controls. Immunohistochemical expression of epithelial markers (E‐cadherin and β‐catenin) was significantly downregulated in periodontitis patients as compared with controls. Periodontitis cases exhibited significant upregulation of Snail1 expression. Furthermore, cytoplasmic vimentin (66.2%) and nuclear β‐catenin (27.7%) were solely expressed in periodontally diseased tissues compared with control. Epithelial markers, E‐cadherin and β‐catenin, were significantly negatively correlated with increasing PPD, while vimentin showed positive correlation with this parameter. Conclusion There were marked downregulation of epithelial molecules and upregulation of mesenchymal markers in gingival tissues derived from periodontitis patients, suggesting expression of the EMT phenotype in the pathological epithelial lining of periodontal pockets.