Human bocavirus detection and quantification in fecal and serum specimens from recipients of allogeneic hematopoietic stem cell transplantation: A longitudinal study

• Published in: Journal of medical virology Vol. 94; no. 2; pp. 594 - 600
• Main Authors: Costa, Brunno Câmara Lopes, Dábilla, Nathânia Alves Silva, Almeida, Tâmera Nunes, Fiaccadori, Fabíola Souza, Souza, Teresinha Teixeira, Cardoso, Divina das Dores de Paula, Arantes, Adriano de Moraes, Souza, Menira
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.02.2022
• Subjects: Adolescent; Adult; Brazil; Deoxyribonucleic acid; Diarrhea; digestive system; DNA; epidemiology; Feces; Feces - virology; Female; Genotype; Genotypes; Hematopoietic Stem Cell Transplantation; Hematopoietic stem cells; Hospitalization; Human bocavirus - genetics; Human bocavirus - isolation & purification; Humans; immune system; Longitudinal Studies; Male; Middle Aged; Parvoviridae Infections - virology; parvovirus; pathogenesis; Polymerase chain reaction; shedding; Stem cell transplantation; Stem cells; Transplantation; viral excretion; Viral Load; Viremia; Viremia - blood; Virology; virus classification; Virus Shedding; Young Adult; Brazil; digestive system; epidemiology; pathogenesis; viral excretion; transplantation; immune system; shedding; virus classification; parvovirus
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/jmv.25486

Objectives The aim of this study was to evaluate the occurrence of human bocavirus (HBoV) and to determine viral loads in samples of patients admitted for allogeneic hematopoietic stem cell transplantation (allo‐HSCT). Methods Fecal and serum samples were collected from 19 patients, during a 24‐month period. Samples were screened by quantitative polymerase chain reaction TaqMan assay, with specific probe and primers targeting the NP1 gene of all HBoVs genotypes (HBoV‐1 to ‐ 4), and viral loads were determined using serial dilutions of a recombinant plasmid. Results HBoV DNA was detected in 42.1% (8 of 19) of the patients in at least one type of sample (feces and/or serum) during the study period, with 75% (6 of 8) of the patients being positive in both types of sample. Viral shedding in feces had a median of 26 days (range, 5 to 121) and viremia was detected in 87.5% (7 of 8) of the patients. The HBoV loads in fecal samples were higher than in sera and, in most cases, HBoV was detected earlier in fecal than in sera samples. In six HBoV‐positive patients (6 of 8) diarrhea was observed concomitantly to viral detection in fecal samples. Conclusions A high frequency and loads of HBoV in allo‐HSCT recipients was observed, especially in fecal samples. Positivity in fecal samples was an early predictor of HBoV presence. Highlight A high postivity for human bocavirus (HBoV) was found in allogeneic hematopoietic stem cell transplantation (allo‐HSCT) recipients. HBoV was detected in fecal and serum samples of allo‐HSCT patients. HBoV in fecal samples was detected earlier than in serum samples. Fecal samples could be used for HBoV monitoring in allo‐HSCT patients.