A long‐term in vitro culture of bovine epithelial cells on collagen rafts

In the present work, we established and characterized a 3D functional polarized primary bovine oviduct epithelial cells (BOECs) culture on free‐floating type I collagen hydrogels (rafts) at an air‐liquid interface (ALI). Intercellular junctions, ultrastructural cellular morphology and the expression...

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Published inReproduction in domestic animals Vol. 56; no. 4; pp. 684 - 687
Main Authors Peña‐Zanoni, Milagros, García, Daniela Celeste, Roldán‐Olarte, Mariela, Valdecantos, Pablo Alberto
Format Journal Article
LanguageEnglish
Published Germany Blackwell Publishing Ltd 01.04.2021
Subjects
3D culture
Biocompatibility
Cell culture
Collagen
Collagen (type I)
Epithelial cells
Epithelium
Homeostasis
Hydrogels
In vivo methods and tests
Mesenchyme
Morphology
Oviduct
Physiology
Rafts
Toxicity
Toxicity testing
epithelial cells
oviduct
3D culture
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Summary:In the present work, we established and characterized a 3D functional polarized primary bovine oviduct epithelial cells (BOECs) culture on free‐floating type I collagen hydrogels (rafts) at an air‐liquid interface (ALI). Intercellular junctions, ultrastructural cellular morphology and the expression of the OVGP1 closely recapitulated those of the in vivo epithelium lining. These morphological and physiological epithelial cell features were maintained under standard DMEM/F12 with 10% foetal bovine serum culture medium for at least 28 days of ALI culture. The versatility of the BOECs raft cultures should allow testing of toxicity compounds, in vitro evaluation of physiological or pathological oviductal states, and the study of epithelial‐mesenchymal interactions that are critical for the maintenance of oviductal homeostasis.
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ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13896