Feasibility of intraperitoneal placental‐derived mesenchymal stem cell injection in stomachs of diabetic mice

• Published in: Journal of gastroenterology and hepatology Vol. 33; no. 6; pp. 1242 - 1247
• Main Authors: Park, Jong Min, Lee, Sang Hwan, Kim, Kwang Il, Kim, Won Hee, Cho, Joo Young, Hahm, Ki Baik, Hong, Sung Pyo
• Format: Journal Article
• Language: English
• Published: Australia Wiley Subscription Services, Inc 01.06.2018
• Subjects: Animal models; Autonomic nervous system; Diabetes; Diabetes mellitus; diabetic gastropathy; Diabetic neuropathy; Injection; Insulin; Interstitial cells; Interstitial cells of Cajal; Mesenchymal stem cells; Mesenchyme; neuronal nitric oxide synthase; Nitric oxide; Nitric-oxide synthase; Placenta; Rodents; Stem cell transplantation; Stomach; neuronal nitric oxide synthase; diabetic gastropathy; mesenchymal stem cells; interstitial cells of Cajal
• ISSN: 0815-9319; 1440-1746
• DOI: 10.1111/jgh.14045

Background and Aim Diabetic gastropathy is associated with loss of interstitial cells of Cajal and autonomic neuropathy. Effective management for diabetic gastropathy is still unavailable. This study was aimed to confirm the pathogenetic changes in diabetic gastropathy and to examine the effect of treatment with placental‐derived mesenchymal stem cells (PDMSCs) in stomachs of animal models. Methods Fourteen non‐obese diabetic/ShiLtJ mice of 8 weeks were bled until week 30. Diabetes mellitus developed in 10 out of 14 mice, which all survived with insulin. The mice were grouped into three groups: nondiabetic group (n = 4), diabetic sham group (n = 5), and diabetic PDMSC group (n = 5) all of which were treated with intraperitoneal PDMSCs injection at week 30. All mice were killed at week 34, and the stomachs were examined by immunohistochemical stain with c‐kit and neuronal nitric oxide synthase antibodies. Results The number of c‐kit positive cells in stomach decreased significantly in the diabetic sham group compared with that in the nondiabetic group (21.2 ± 6.7 vs 88.0 ± 29.3, P = 0.006) but increased with PDMSC treatment (21.2 ± 6.7 vs 64.0 ± 15.1, P = 0.02). The positive rate of neuronal nitric oxide synthase in neural plexus was also significantly lower in the diabetic sham group than in the nondiabetic group (22.3% ± 18.5% vs 48.0% ± 22.7%, P = 0.003) but increased with PDMSC treatment (22.3% ± 18.5% vs 43.3% ± 20.5%, P = 0.03). Conclusions Interstitial cells of Cajal and neural plexus decreased in stomachs of mice with diabetes mellitus but were significantly repaired with intraperitoneal injection of PDMSC.