Integrated serum proteomic and N‐glycoproteomic characterization of dengue patients

Dengue fever is a mosquito‐borne viral disease caused by the dengue virus (DENV). It poses a public health threat globally and, while most people with dengue have mild symptoms or are asymptomatic, approximately 5% of affected individuals develop severe disease and need hospital care. However, knowl...

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Published inJournal of medical virology Vol. 96; no. 7; pp. e29775 - n/a
Main Authors Hu, Xiao, Song, Jiamin, Ye, Guoguo, Zhu, Miao, Lan, Jianfeng, Zeng, Lijiao, Ke, Zhiyi, Yuan, Jing
Format Journal Article
LanguageEnglish
Published United States Wiley Subscription Services, Inc 01.07.2024
Subjects
Adult
Antibiotics
Aquatic insects
Dengue - blood
Dengue - virology
Dengue fever
Dengue Virus
Female
Glycan
Glycopeptides
Glycoproteins
Glycoproteins - blood
Glycosylation
Health risks
Humans
Infections
intact N‐glycopeptide
Male
Middle Aged
Molecular modelling
N‐glycoproteomic
Pathogenesis
Proteome - analysis
proteomic
Proteomics
Proteomics - methods
Public health
Vector-borne diseases
Viral diseases
proteomic
dengue fever
N‐glycoproteomic
intact N‐glycopeptide
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Summary:Dengue fever is a mosquito‐borne viral disease caused by the dengue virus (DENV). It poses a public health threat globally and, while most people with dengue have mild symptoms or are asymptomatic, approximately 5% of affected individuals develop severe disease and need hospital care. However, knowledge of the molecular mechanisms underlying dengue infection and the interaction between the virus and its host remains limited. In the present study, we performed a quantitative proteomic and N‐glycoproteomic analysis of serum from 19 patients with dengue and 11 healthy people. The results revealed distinct proteomic and N‐glycoproteomic landscapes between the two groups. Notably, we report for the first time the changes in the serum N glycosylation pattern following dengue infection and provide abundant information on glycoproteins, glycosylation sites, and intact N‐glycopeptides using recently developed site‐specific glycoproteomic approaches. Furthermore, a series of key functional pathways in proteomic and N‐glycoproteomic were identified. Collectively, our findings significantly improve understanding of host and DENV interactions and the general pathogenesis and pathology of DENV, laying a foundation for functional studies of glycosylation and glycan structures in dengue infection.
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ISSN:0146-6615
1096-9071
1096-9071
DOI:10.1002/jmv.29775