Betanodavirus infection in bath‐challenged Solea senegalensis juveniles: A comparative analysis of RGNNV, SJNNV and reassortant strains

• Published in: Journal of fish diseases Vol. 41; no. 10; pp. 1571 - 1578
• Main Authors: Souto, Sandra, Olveira, José G., Alonso, M. Carmen, Dopazo, Carlos P., Bandín, Isabel
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.10.2018
• Subjects: Animals; betanodavirus; Brain; Brain - pathology; Brain - virology; Comparative analysis; Disease Susceptibility - virology; Fish; Fish Diseases - epidemiology; Fish Diseases - mortality; Fish Diseases - pathology; Fish Diseases - virology; Fish skin; Flatfishes - anatomy & histology; Flatfishes - virology; Genotype; Genotypes; Gills; Gills - virology; Infections; Infectivity; Juveniles; Microbiological strains; Mouth - virology; Nodaviridae - genetics; Nodaviridae - isolation & purification; Nodaviridae - pathogenicity; Nodaviridae - physiology; reassortant; Reassortant Viruses - genetics; Reassortant Viruses - isolation & purification; Replication; RGNNV; RNA Virus Infections - epidemiology; RNA Virus Infections - mortality; RNA Virus Infections - pathology; RNA Virus Infections - virology; RNA, Viral - genetics; senegalese sole; SJNNV; Skin - virology; Strains (organisms); Tissue; Viral Load; viral replication; Virulence; Virus Internalization; Virus Replication; viral replication; SJNNV; betanodavirus; RGNNV; reassortant; senegalese sole
• ISSN: 0140-7775; 1365-2761
• DOI: 10.1111/jfd.12865

Senegalese sole has been shown to be highly susceptible to betanodavirus infection, although virulence differences were observed between strains. To study the mechanisms involved in these differences, we have analysed the replication in brain tissue of three strains with different genotypes during 15 days after bath infection. In addition, possible portals of entry for betanodavirus into sole were investigated. The reassortant RGNNV/SJNNV and the SJNNV strain reached the brain after 1 and 2 days postinfection, respectively. Although no RGNNV replication was detected until day 3–4 postinfection, at the end of the experiment this strain yielded the highest viral load; this is in accordance with previous studies in which sole infected with the reassortant showed more acute signs and earlier mortality than the RGNNV and SJNNV strains. Differences between strains were also observed in the possible portals of entry. Thus, whereas the reassortant strain could infect sole mainly through the skin or the oral route, and, to a minor extent, through the gills, the SJNNV strain seems to enter fish only through the gills and the RGNNV strain could use all tissues indistinctly. Taken together, all these results support the hypothesis that reassortment has improved betanodavirus infectivity for sole.