Molecular and Physiological Properties of Indigenous Strains of Oenococcus oeni Selected from Nero di Troia Wine (Apulia, Italy)

• Published in: Microorganisms (Basel) Vol. 10; no. 4; p. 795
• Main Authors: Cappello, Maria Stella, Falco, Vittorio, Curcio, Rosita, Mita, Giovanni, Zapparoli, Giacomo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09.04.2022; MDPI
• Subjects: Acetaldehyde; Acids; Amplified fragment length polymorphism; amplified fragments length polymorphisms; Bacteria; Citric acid; Cluster analysis; enzymatic activities; Enzymatic activity; Esterase; Genetic diversity; Genomics; Genotypes; Glucosidase; Malic acid; metabolic properties; Metabolism; Nero di Troia wine; Oenococcus oeni; Physiology; Polymorphism; Population structure; Populations; Principal components analysis; Regions; Starters; Strains (organisms); Variance analysis; Wine; Wineries & vineyards; β-Glucosidase; United States > US; Italy; Germany; Nero di Troia wine; genetic diversity; amplified fragments length polymorphisms; Oenococcus oeni; enzymatic activities; metabolic properties
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms10040795

The characterization of Oenococcus oeni strains isolated from Nero di Troia wine (Apulia, Italy) sampled in two distinct production areas was carried out. The two indigenous populations, consisting of 95 and 97 isolates, displayed high genetic diversity when analyzed by amplified fragments length polymorphisms (AFLP). Based on the UPGMA dendrogram obtained by AFLP analysis, the two populations displayed similar genotypes that grouped in the same clusters with a high level of similarity (>95%). One genotype was found in only one of the two areas. Representative strains of each cluster were analyzed for their enzymatic activities (esterase, β-glucosidase, and protease), assayed in whole cells, and tested for their metabolic properties (consumption of L-malic acid, citric acid, acetaldehyde, and arginine) and growth parameters. Significant differences among strains, including the reference strain ATCC BAA-1163, were observed for all of these properties. Principal component analysis evidenced phenotypic differences among strains, and well separated some of them belonging to different genotypes. Strains exhibiting the best performances in most of these traits could be further investigated in order to select possible candidates as malolactic starters for Nero di Troia wine. This study provided insights on the population structure of O. oeni of a local winemaking area useful to the understanding of the regional diversity of this bacterium, an issue not yet completely resolved