Role of psoriatic keratinocytes in the metabolic reprogramming of dermal mesenchymal stem cells

• Published in: International journal of dermatology Vol. 61; no. 3; pp. 337 - 345
• Main Authors: Cao, Yue, Liang, Nan‐Nan, Chang, Wen‐Juan, Li, Jun‐Qin, Jiao, Juan‐Juan, Hou, Rui‐Xia, Li, Juan, Zhang, Kai‐Ming
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.03.2022
• Subjects: Abnormalities; Cell Proliferation; Cells, Cultured; Coculture Techniques; Epidermal growth factor; Glucose transporter; Glycolysis; Growth factors; Humans; Keratinocytes; Mesenchymal Stem Cells; Metabolism; Mitochondria; Myc protein; Pathogenesis; Psoriasis; Skin diseases; Stem cell factor; Stem cells; Stimulation; Western blotting
• ISSN: 0011-9059; 1365-4632; 1365-4632
• DOI: 10.1111/ijd.15855

Background Psoriasis is an immune‐mediated inflammatory skin disease, featured by epidermal hyperproliferation. Psoriasis exhibits metabolic abnormalities, which can further aggravate the condition of psoriasis. The present study aimed to investigate the role of psoriatic keratinocytes (KCs) in the metabolic reprogramming of dermal mesenchymal stem cells (DMSCs). Methods Dermal mesenchymal stem cells were cocultured with primary KCs either from psoriatic lesions or from normal subjects using Transwell plate. Glycolysis and mitochondrial metabolism of DMSCs were detected by Seahorse Metabolic Analyzer. Expression levels of proteins were analyzed by Western blotting. DMSCs proliferation was assessed using 5‐ethynyl‐2′‐deoxyuridine assay and Cell Counting Kit‐8. Results In comparison with normal KCs, coculture of psoriatic KCs with DMSCs dramatically increased glycolytic and mitochondrial metabolism, and expression levels of stem cell factor, epidermal growth factor, glucose transporter 1, and c‐Myc. Moreover, psoriatic KCs were more potent than normal KCs in the stimulation of DMSC proliferation. Conclusions In conclusion, psoriatic KCs display a higher potency in metabolic reprogramming and stimulation of DMSC proliferation, possibly contributing to the pathogenesis of psoriasis. However, whether the intervention of metabolic reprogramming of DMSCs can alleviate psoriasis remains to be determined.