Bone morphogenetic protein 15 supplementation enhances cumulus expansion, nuclear maturation and progesterone production of in vitro‐matured bovine cumulus‐oocyte complexes

In vitro embryo production (IVP) efficiency is reduced when compared to in vivo. The basic knowledge of bovine in vitro oocyte maturation (IVM) mechanisms provides support to improve in vitro embryo production yields. The present study assessed the effects of bone morphogenetic protein 15 (BMP15), f...

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Published inReproduction in domestic animals Vol. 56; no. 5; pp. 754 - 763
Main Authors Delgado, Juliana de Carvalho, Hamilton, Thais Rose dos Santos, Mendes, Camilla Mota, Siqueira, Adriano Felipe Perez, Goissis, Marcelo Demarchi, Buratini, José, Assumpção, Mayra Elena Ortiz D’Ávila
Format Journal Article
LanguageEnglish
Published Germany Blackwell Publishing Ltd 01.05.2021
Subjects
Deoxyribonucleic acid
DNA
DNA fragmentation
Embryos
Expansion
FGF16
Fibroblast growth factors
Fragmentation
Gametocytes
glucose consumption
Growth factors
in vitro maturation
IVM medium
lactate production
Maturation
Metabolism
oocyte DNA fragmentation
Oocytes
Progesterone
Proteins
Supplements
FGF16
glucose consumption
in vitro maturation
lactate production
IVM medium
oocyte DNA fragmentation
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Summary:In vitro embryo production (IVP) efficiency is reduced when compared to in vivo. The basic knowledge of bovine in vitro oocyte maturation (IVM) mechanisms provides support to improve in vitro embryo production yields. The present study assessed the effects of bone morphogenetic protein 15 (BMP15), fibroblast growth factor 16 (FGF16) and their combined action on cumulus cells (CC) expansion, oocyte and CC DNA fragmentation, oocyte nuclear maturation, energetic metabolism and progesterone production in bovine IVM. Cumulus‐oocyte complexes (COC) were matured in control or supplemented media containing BMP15 (100 ng/ml), FGF16 (10 ng/ml) or BMP15 combined with FGF16; and assessed at 0 and 22 hr of IVM. BMP15 alone or its association with FGF16 enhanced cumulus expansion. BMP15 decreased DNA fragmentation in both CC and oocytes, and improved oocyte nuclear maturation rate. In addition, BMP15 increased CC progesterone production, an effect not previously reported. The present study reinforces previous data pointing to a beneficial influence of BMP15 during IVM, while providing novel evidence that the underlying mechanisms involve increased progesterone production.
Bibliography:Delgado and Hamilton contributed equally.
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ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13914