Omega‐3‐rich Isochrysis sp. biomass enhances brain docosahexaenoic acid levels and improves serum lipid profile and antioxidant status in Wistar rats

• Published in: Journal of the science of food and agriculture Vol. 99; no. 13; pp. 6066 - 6075
• Main Authors: Balakrishnan, Jeyakumar, Dhavamani, Sugasini, Sadasivam, Selvam Govindan, Arumugam, Muthu, Vellaikumar, Sampathrajan, Ramalingam, Jagadeesan, Shanmugam, Kathiresan
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.10.2019; John Wiley and Sons, Limited
• Subjects: Animal models; Animal tissues; Animals; Antioxidants; Antioxidants - metabolism; Biomass; Brain; Brain - metabolism; Creatinine; desaturase; DHA; Dietary supplements; Dietary Supplements - analysis; Docosahexaenoic acid; Docosahexaenoic Acids - administration & dosage; Docosahexaenoic Acids - metabolism; Eicosapentaenoic acid; Eicosapentaenoic Acid - administration & dosage; Eicosapentaenoic Acid - analogs & derivatives; Eicosapentaenoic Acid - metabolism; EPA; Fatty acids; Fatty Acids, Unsaturated - metabolism; Fish oils; Gene Expression; Genes; Haptophyta - chemistry; Haptophyta - growth & development; Haptophyta - metabolism; High density lipoprotein; Inflammation; Isochrysis; Isochrysis sp; Lipids; Lipids - blood; liver; Liver - metabolism; Male; Markers; Microalgae - chemistry; Microalgae - growth & development; Microalgae - metabolism; Oils & fats; Organic chemistry; Organs; Oxidative stress; Polyunsaturated fatty acids; Rats; Rats, Wistar; Rodents; Toxicity; Urea; Wistar rat; Isochrysis sp; desaturase; Wistar rat; DHA; liver; EPA
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.9884

BACKGROUND Isochrysis sp. is a marine microalga, rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The potential use of its biomass as an alternative source of polyunsaturated fatty acids (PUFAs) has not been studied in animal models. Male albino Wistar rats were divided into three groups and treated for 28 days. The rats were fed with (1) standard chow (control group), (2) microalgal biomass rich in EPA and DHA along with standard chow (microalga group), and (3) fish oil that contains equivalent amounts of EPA and DHA along with standard chow (fish oil group). After intervention, biochemical indices, histopathological indices, relative mRNA expression of PUFA genes, antioxidant genes, inflammatory markers, and the fatty acid profile of major tissues were studied. RESULTS Animals treated with microalgal biomass showed significantly increased serum HDL levels (P < 0.05) and reduced oxidative stress markers with a concomitant decrease in urea and creatinine levels. Oral supplementation of microalgal biomass did not show any toxicity or damage in any major organs. The mRNA expression of PUFA genes was significantly downregulated (P < 0.05) and antioxidant genes were upregulated. Furthermore, the mRNA expression of pro‐inflammatory markers was significantly downregulated (P < 0.05) and anti‐inflammatory markers were upregulated. Oral supplementation of microalgal biomass improved DHA status in brain and liver. CONCLUSION The present study demonstrated that Isochrysis sp. can be used as a safe, alternative food supplement for ω‐3 fatty acids. © 2019 Society of Chemical Industry