Neutrophils induce smooth muscle hyperplasia via neutrophil elastase‐induced FGF‐2 in a mouse model of asthma with mixed inflammation
Summary Background Bronchial asthma is traditionally characterized by chronic allergic inflammation, including eosinophilia and elevated Th2 cytokines. Recently, IL‐17‐derived neutrophil infiltration was shown to correlate with asthma severity and airway remodelling. Objective To investigate the rol...
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Published in | Clinical and experimental allergy Vol. 48; no. 12; pp. 1715 - 1725 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Wiley Subscription Services, Inc
01.12.2018
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Subjects | |
Online Access | Get full text |
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Summary: | Summary
Background
Bronchial asthma is traditionally characterized by chronic allergic inflammation, including eosinophilia and elevated Th2 cytokines. Recently, IL‐17‐derived neutrophil infiltration was shown to correlate with asthma severity and airway remodelling.
Objective
To investigate the role of IL‐17‐derived neutrophils in airway remodelling in chronic bronchial asthma.
Methods
We utilized house dust mite antigen‐induced mouse models of asthma. Intranasal sensitization and chronic antigen challenge caused a mixed allergic inflammation that included eosinophils and neutrophils (Mix‐in group). We neutralized IL‐17 and fibroblast growth factor (FGF‐2) and investigated the mechanism of airway remodelling in the Mix‐in group.
Results
The Mix‐in group displayed neutrophilic infiltration and high levels of IL‐17 in lung tissue. The Mix‐in group also exhibited more bronchial smooth muscle hyperplasia. IL‐17 neutralization decreased the magnitude of all of these effects in the Mix‐in group. Antibody arrays revealed an increase in FGF‐2 in the Mix‐in Group relative to the Eo‐ip group, and FGF‐2 elevation was associated with smooth muscle hypertrophy/hyperplasia. High concentrations of neutrophil elastase enhanced E‐cadherin/β‐catenin signalling in bronchial epithelial cells. Neutrophil elastase inhibitor treatment decreased FGF‐2 production and E‐cadherin/β‐catenin signalling, which inhibited smooth muscle hyperplasia.
Conclusion
The IL‐17/neutrophil axis may play an important role in airway remodelling by contributing to smooth muscle hypertrophy/hyperplasia in mixed allergic inflammation and accordingly represents an attractive therapeutic target for severe asthma. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0954-7894 1365-2222 |
DOI: | 10.1111/cea.13263 |