Allelic variation of PmCBF03 contributes to the altitude and temperature adaptability in Japanese apricot (Prunus mume Sieb. et Zucc.)

Japanese apricot is an important subtropical deciduous fruit tree in China, widely distributed in different altitude areas. How does it adapt to the different temperature environments in these areas? In this study, we identified a low‐temperature transcription factor PmCBF03 on chromosome 7 through...

Full description

Saved in:
Bibliographic Details
Published inPlant, cell and environment Vol. 47; no. 4; pp. 1379 - 1396
Main Authors Huang, Xiao, Gao, Feng, Zhou, Pengyu, Ma, Chengdong, Tan, Wei, Ma, Yufan, Li, Minglu, Ni, Zhaojun, Shi, Ting, Hayat, Faisal, Li, Yongping, Gao, Zhihong
Format Journal Article
LanguageEnglish
Published United States Wiley Subscription Services, Inc 01.04.2024
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:Japanese apricot is an important subtropical deciduous fruit tree in China, widely distributed in different altitude areas. How does it adapt to the different temperature environments in these areas? In this study, we identified a low‐temperature transcription factor PmCBF03 on chromosome 7 through adaptive analysis of populations at different altitudes, which has an early termination single nucleotide polymorphism mutation. There were two different types of variation, PmCBF03A type in high‐altitude areas and PmCBF03T type in low‐altitude areas. PmCBF03A gene increased the survival rate, Fv/Fm values, antioxidant enzyme activity, and expression levels of antioxidant enzyme genes, and reducing electrolyte leakage and accumulation of reactive oxygen species in transgenic Arabidopsis under low temperature and freezing stress. Simultaneously, PmCBF03A gene promoted the dormancy of transgenic Arabidopsis seeds than wild‐type. Biochemical analysis demonstrated that PmCBF03A directly bound to the DRE/CRT element in the promoters of the PmCOR413, PmDAM6 and PmABI5 genes, promoting their transcription and enhanced the cold resistance and dormancy of the overexpressing PmCBF03A lines. While PmCBF03T gene is unable to bind to the promoters of PmDAM6 and PmABI5 genes, leading to early release of dormancy to adapt to the problem of insufficient chilling requirement in low‐altitude areas. Summary statement The PmCBF03 allele variation is involved in regulating the low‐temperature adaptability of Japanese apricots at different altitudes.
Bibliography:Xiao Huang, Feng Gao, and Pengyu Zhou contributed equally to this work.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0140-7791
1365-3040
DOI:10.1111/pce.14813