Olfactory co‐receptor is involved in host recognition and oviposition in Ophraella communa (Coleoptera: Chrysomelidae)

• Published in: Insect molecular biology Vol. 29; no. 4; pp. 381 - 390
• Main Authors: Ma, Chao, Cui, Shaowei, Bai, Qiang, Tian, Zhenya, Zhang, Yan, Chen, Guangmei, Gao, Xuyuan, Tian, Zhenqi, Chen, Hongsong, Guo, Jianying, Wan, Fanghao, Zhou, Zhongshi
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.08.2020; Blackwell Publishing Ltd
• Subjects: Ambrosia - growth & development; Amino Acid Sequence; Animals; Beetles; behavioural test; Coleoptera; Coleoptera - genetics; Coleoptera - physiology; co‐receptor; electroantennogram; Females; Food Chain; Gene expression; Hatching; Insect Proteins - chemistry; Insect Proteins - genetics; Insect Proteins - metabolism; Insects; Invasive plants; Molecular modelling; odorant receptor; Olfaction; Olfactory perception; Olfactory system; Ophraella communa; Oviposition; Oviposition - genetics; Phylogeny; Receptors; Receptors, Odorant - chemistry; Receptors, Odorant - genetics; Receptors, Odorant - metabolism; Recognition; reproduction; RNA interference; RNA-mediated interference; Sequence Alignment; Smell; Volatiles; electroantennogram; co-receptor; RNA interference; behavioural test; odorant receptor; Ophraella communa; reproduction
• ISSN: 0962-1075; 1365-2583
• DOI: 10.1111/imb.12643

Common ragweed (Ambrosia artemisiifolia) is a notorious invasive weed that has spread across most temperate regions of the world. The beetle (Ophraella communa) is considered to be an effective control agent against A. artemisiifolia. As an oligophagous insect, its olfactory system is extremely important for host seeking in the wild. To the best of our knowledge, there is no report on the molecular mechanisms underlying olfaction recognition in this beetle. Hence, in this study, we characterized the odorant receptor co‐receptor of O. communa and named it as ‘OcomORco’. Real‐time quantitative PCR (qRT‐PCR) showed that, compared to the control treatment, RNA interference (RNAi) strongly reduced the expression of OcomORco by 89% in male and 90% in female beetles. Electroantennogram assay showed that the antennal response of both male and female beetles to four volatiles of A. artemisiifolia was significantly reduced. The injected male or female beetles lost their preference for plant leaves as observed in the behavioural tests. In addition, disruption of the expression of OcomORco resulted in a reduction of oviposition, while there was no difference in larval hatching rate between control and knockdown females. We demonstrated that OcomORco plays a vital role in olfactory perception and host search in O. communa, and it is involved in oviposition in an indirect way. Olfactory co‐receptor (ORco) of Ophraella communa was first characterized. Antennal response of RNAi‐treated beetles to volatiles of A. artemisiifolia was significantly reduced and they lost preference to plant leaves. Disruption of the expression of OcomORco in female beetles resulted in a reduction of oviposition.