Genotoxic and mutagenic effects of methyl ether dillapiole on the development of Aedes aegypti (Diptera: Culicidae)

• Published in: Medical and veterinary entomology Vol. 35; no. 4; pp. 556 - 566
• Main Authors: da Silva, J. S., da Silva Pinto, A. C., dos Santos, L. H. F., da Silva, L. J. S., da Cruz, D. L. V., Rafael, M. S.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.12.2021; Wiley Subscription Services, Inc
• Subjects: Aedes - genetics; Aedes aegypti; Allyl Compounds; Animals; Chikungunya; Chikungunya virus; chromosomes; Culicidae; dengue fever; Developmental stages; Dioxoles; DNA Damage; DNA fragmentation; Essential oils; Female; Genomes; Genotoxicity; genotoxocity; Hatching; Insecticides; Insecticides - pharmacology; Larva; Methyl Ethers - pharmacology; mutagenesis; Mutagens - pharmacology; Neuroblasts; Oocytes; Oviposition; Temefos - pharmacology; vector control; zika; dengue fever; chromosomes; genotoxocity; Chikungunya; zika; mutagenesis; vector control
• ISSN: 0269-283X; 1365-2915
• DOI: 10.1111/mve.12533

Dillapiole, extracted from Piper aduncum essential oil and its derivatives, has been shown to be a potential alternative to the control of Aedes aegypti, which has become resistant to synthetic insecticides. Methyl ether dillapiole (MED) and temephos (TM) were compared to complement the data on the genotoxicity and developmental changes of Ae. aegypti. Over four generations (G1–G4), third stage larvae were treated with MED at 60, 80 and 100 μg/mL and TM at 0.002, 0.005 and 0.007 μg/mL for 4 h. Adult females were separated to estimate oviposition and hatching rates, and total egg length. Over the four generations, a significant reduction was recorded in oviposition and hatching rates, and in mean egg length (Tukey, P < 0.05), compared with the negative control (NC). Cytological slide preparations were done from adult oocytes and larval neuroblasts. The cumulative effects of genotoxic (bridges, budding and nuclear fragmentation) and mutagenic (micronucleus and chromosomal breakage) damage was observed in the neuroblasts and oocytes of exposed mosquitoes. Developmental changes and damage to the genome of MED‐treated Ae. aegypti were greater than those caused by TM. Further studies should focus on understanding the effects of the MED molecule on Ae. aegypti. In this study methyl ether dillapiole (MED) and temephos (TM) were tested for four generations (G1–G4) to check on the genotoxicity and developmental changes of Ae. aegypti. The MED at 60, 80 and 100 μg/mL and TM at 0.002, 0.005 and 0.007 μg/mL reduced oviposition, hatching and average egg length of Ae. aegypti. MED caused demage of genotoxic and mutagenic in the neuroblasts and oocytes of Ae. aegypti exposed for 4 h, presenting potential effect for the control of mosquitoes.