Comparison of adipophilin and recently introduced PReferentially expressed Antigen in MElanoma immunohistochemistry in the assessment of sebaceous neoplasms: A pilot study

• Published in: Journal of cutaneous pathology Vol. 48; no. 10; pp. 1252 - 1261
• Main Authors: Donnell, Sarah A., LeBlanc, Robert E., Yan, Shaofeng, Parra, Ourania, Momtahen, Shabnam, Sriharan, Aravindhan, Linos, Konstantinos
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.10.2021; Wiley Subscription Services, Inc
• Subjects: adenoma; adipophilin; Aged; Aged, 80 and over; Antigens; Antigens, Neoplasm - analysis; Antigens, Neoplasm - biosynthesis; Biomarkers, Tumor - analysis; carcinoma; Female; Humans; Immunohistochemistry; Male; Melanoma; Middle Aged; Perilipin-2 - analysis; Perilipin-2 - biosynthesis; Pilot Projects; PRAME; sebaceous; Sebaceous gland; Sebaceous Gland Neoplasms - diagnosis; Tumors; PRAME; sebaceous; adenoma; carcinoma; adipophilin
• ISSN: 0303-6987; 1600-0560
• DOI: 10.1111/cup.14043

Background We and others have noticed consistent staining of sebaceous glands with PReferentially expressed Antigen in MElanoma (PRAME). We aimed to determine whether PRAME was as sensitive, specific, and interpretable as adipophilin for distinguishing sebaceous neoplasms (SNs) from other neoplasms. Methods Twenty SNs and 32 control cases were stained for PRAME and adipophilin. Extent of staining was scored as follows: 0, no staining; 1, <5% positivity; 2, 5% to 50% positivity; and 3, >50% positivity. Intensity was scored as negative, weak, moderate, or strong. A composite score was determined by adding the scores for extent and intensity. Results PRAME had positive composite scores in all 20 SNs in the more differentiated areas, whereas adipophilin had positive composite scores in 19/20 cases. PRAME showed positivity in the basaloid cells in 15/16 cases, whereas adipophilin was positive in 14. Among controls, PRAME and adipophilin had positive composite scores in 3/32 cases and 6/32 cases, respectively. Conclusions PRAME and adipophilin are comparable in terms of distribution and intensity for staining sebocytes. In the basaloid cells, PRAME expression is often more diffuse and easier to detect than adipophilin. In comparing the SNs to the controls, PRAME was more sensitive and more specific than adipophilin. PRAME could be used as an additional marker of sebaceous differentiation in everyday practice.