Protective effect of microRNA‐134‐3p on multiple sclerosis through inhibiting PRSS57 and promotion of CD34+ cell proliferation in rats

• Published in: Journal of cellular biochemistry Vol. 121; no. 11; pp. 4347 - 4363
• Main Authors: Song, Qihan, Zhao, Fengli, Yao, Jingfan, Dai, Hailin, Hu, Lei, Yu, Shun
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.11.2020
• Subjects: Apoptosis; Assaying; CD34 antigen; CD34+ cells; Cell growth; Cell proliferation; Flow cytometry; Inflammation; Inflammatory response; Interleukins; Membrane potential; MicroRNAs; microRNA‐134‐3p; miRNA; Mitochondria; Multiple sclerosis; Polymerase chain reaction; proliferation; PRSS57; Reporter gene; Reverse transcription; Ribonucleic acid; RNA; Serine; Serine proteinase; Spinal cord; apoptosis; microRNA-134-3p; PRSS57; proliferation; multiple sclerosis; CD34+ cells
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.29643

MicroRNAs (miRs) have been extensively studied for their involvement in multiple sclerosis (MS). We investigated the involvement of miR‐134‐3p on MS. The MS rat model was established, and positive expression of interleukin‐17 (IL‐17) was detected using the immunohistochemical method while the expression of miR‐134‐3p and serine protease 57 (PRSS57) was determined by means of reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) and Western blot analysis. Second, the miR‐134‐3p overexpression or short hairpin RNA against PRSS57 was introduced into the CD34+ cells to investigate the levels of proliferation and apoptosis‐related genes by RT‐qPCR and Western blot analysis. In addition, analysis of the targeting relations of miR‐134‐3p and PRSS57 was conducted using online software and dual‐luciferase reporter gene assay. Furthermore, neuronal functions, inflammatory response, proliferation, and apoptosis of CD34+ cells were assayed by flow cytometry, enzyme‐linked immunosorbent assay, and methyl thiazolyl tetrazolium. IL‐17 and PRSS57 expression increased while miR‐134‐3p expression decreased in the spinal cord from MS rats. miR‐134‐3p could target PRSS57. miR‐134‐3p overexpression or PRSS57 silencing enhanced mitochondrial activity of neurons, mitochondrial membrane potential content, CD34+ cell proliferation, while decreasing Cyt C content, inflammatory response, and cell apoptosis. Collectively, overexpression of miR‐134‐3p promotes CD34+ cell proliferation via inhibition of PRSS57 in MS, which may serve as a promising target for MS intervention. Collectively, overexpression of microRNA‐134‐3p promotes CD34+ cell proliferation via inhibition of protease 57 in multiple sclerosis (MS), which may serve as a promising target for MS intervention.