circRNA_0058097 promotes tension‐induced degeneration of endplate chondrocytes by regulating HDAC4 expression through sponge adsorption of miR‐365a‐5p

• Published in: Journal of cellular biochemistry Vol. 121; no. 1; pp. 418 - 429
• Main Authors: Xiao, Liang, Ding, Baiyang, Xu, Shujuan, Gao, Jianming, Yang, Bijing, Wang, Jing, Xu, Hongguang
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.01.2020
• Subjects: Actins - metabolism; Adsorption; Cartilage; Cartilage - metabolism; Chondrocytes; Chondrocytes - cytology; Chondrocytes - metabolism; circRNA; Degeneration; endplate chondrocytes; Extracellular matrix; Extracellular Matrix - metabolism; Gene expression; Histone deacetylase; Histone Deacetylases - metabolism; Humans; In Situ Hybridization, Fluorescence; intermittent cyclic mechanical tension; Intervertebral Disc - metabolism; Intervertebral Disc Degeneration - metabolism; MicroRNAs - metabolism; Repressor Proteins - metabolism; Ribonucleic acid; RNA; RNA - metabolism; RNA, Circular - genetics; Signal Transduction; Stress, Mechanical; Tension; endplate chondrocytes; circRNA; degeneration; intermittent cyclic mechanical tension
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.29202

Excessive mechanical tension can lead to the degeneration of endplate chondrocytes. The presence of tension‐sensitive circRNA_0058097 molecules has been detected in human endplate chondrocytes, where it was found to be a potential competing endogenous RNA. Indeed, inhibiting the expression of circRNA_0058097 effectively enhanced the stress resistance of endplate chondrocytes, suggesting that it may be an important trigger point for the degeneration of endplate cartilage. Through a series of experiments, we reveal that circRNA_0058097 can upregulate the expression of downstream target gene histone deacetylase 4 by sponge adsorption of miR‐365a‐5p, which promoted morphological changes of endplate chondrocytes, and increased extracellular matrix degradation and degeneration of endplate cartilage. Therefore, circRNA_0058097 may provide a new way to prevent and treat endplate cartilage degeneration. Excessive intermittent cyclic mechanical tension can induce the degeneration of endplate chondrocytes. Tension‐sensitive circRNA_0058097 can upregulate the expression of downstream target gene histone deacetylase 4 by sponge adsorption of miR‐365a‐5p, which promoted morphological changes of endplate chondrocytes, and increased extracellular matrix degradation and degeneration of endplate cartilage.