Synthesis of a novel nanocomposite containing chitosan as a three‐dimensional printed wound dressing technique: Emphasis on gene expression

• Published in: Biotechnology progress Vol. 37; no. 4; pp. e3132 - n/a
• Main Authors: Azadmanesh, Fatemeh, Pourmadadi, Mehrab, Zavar Reza, Javad, Yazdian, Fatemeh, Omidi, Meisam, Haghirosadat, Bibi Fatemeh
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.07.2021; Wiley Subscription Services, Inc
• Subjects: Acids; Ammonium; Antibacterial activity; Biocompatibility; Chitosan; Citric acid; Copper; copper carbon dots; Cytotoxicity; Gene expression; Gram-negative bacteria; Hyaluronic acid; Hydrogels; Lactic acid; Nanocomposites; Platelet-derived growth factor; Regeneration; Rosmarinic acid; Scaffolds; Stem cell transplantation; Stem cells; Three dimensional printing; three‐dimensional bioprinting; Toxicity; Wound healing; three-dimensional bioprinting; copper carbon dots; wound healing
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1002/btpr.3132

In this study, a highly porous three‐dimensional (3D)‐printed wound healing core/shell scaffold fabricated using poly‐lactic acid (PLA). The core of scaffold was composed of hyaluronic acid (HA), copper carbon dots (Cu‐CDs), rosmarinic acid, and chitosan hydrogel. Cu‐CDs were synthesized using ammonium hydrogen citrate under hydrothermal conditions. Formulation containing 1 mg ml−1concentration of Cu‐CDs showed an excellent antibacterial activity against gram bacteria. At 0.25 mg ml−1 of Cu‐CDs concentration, scaffold had a good biocompatibility as confirmed by cytotoxicity assay on L929 fibroblast stem cells. in vivo wound healing experiments on groups of rats revealed that after 15 days of treatment, the optimal formulation of composite scaffold significantly improves the wound healing process compared to the PLA scaffold. This finding was confirmed by histological analysis and the relative expression of PDGF, TGF‐β, and MMP‐1 genes. The biocompatible antibacterial CU‐CDS/PLA/HA/chitosan/rosmarinic acid nanocomposite is a promising wound healing scaffold which highly accelerates the process of skin regeneration.