A bacterium with close genetic identity to Pseudomonas mandelii associated with spring fish kills in wild bluegill Lepomis macrochirus Rafinesque and pumpkinseed sunfish Lepomis gibbosus (Linnaeus)

• Published in: Journal of fish diseases Vol. 40; no. 12; pp. 1757 - 1764
• Main Authors: Lovy, J, Dicarlo‐Emery, D, Hutcheson, J M
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.12.2017
• Subjects: Animals; Aquatic environment; Bacteremia; Bacteria; Cultures; Deoxyribonucleic acid; DNA; DNA Gyrase - genetics; DNA topoisomerase; DNA, Bacterial - genetics; Fish; Fish diseases; Fish Diseases - microbiology; Freshwater fishes; Gene sequencing; Genetic analysis; gyrB; Immunosuppression; Kidney - microbiology; Kidneys; Lepomis gibbosus; Lepomis macrochirus; Mass mortality; New Jersey; Nucleotide sequence; Perciformes - microbiology; Pseudomonas; Pseudomonas - classification; Pseudomonas - genetics; Pseudomonas - isolation & purification; Pseudomonas - physiology; Pseudomonas fluorescens; Pseudomonas Infections - veterinary; Pseudomonas mandelii; RNA, Ribosomal, 16S - genetics; rpoD; rRNA 16S; Species; Spring; sunfish; Temperature; Water temperature; gyrB; bacteria; Pseudomonas mandelii; sunfish; rpoD
• ISSN: 0140-7775; 1365-2761
• DOI: 10.1111/jfd.12642

Pseudomonas fluorescens are known bacterial pathogens in fish. The P. fluorescens group contains at least nine different bacterial species, although species from fish have rarely been differentiated. Two isolated fish kills affecting wild bluegills, Lepomis macrochirus Rafinesque, and pumpkinseed sunfish, Lepomis gibbosus (Linnaeus), occurred in the spring of 2015 during cool water temperatures (12.5°C–15.5°C). Disease signs included severe bacteraemia with rare gross external signs. Pure bacterial cultures isolated from kidneys of all affected fish were identified as P. fluorescens using the API 20NE system, while no bacteria were isolated from asymptomatic fish. To further identify the species of bacterium within the P. fluorescens complex, genetic analysis of the 16S rRNA, rpoD and gyrB genes was conducted. DNA sequences of bacterial isolates from both mortality events were identical and had close identity (≥99.7%) to Pseudomonas mandelii. Although likely widespread in the aquatic environment, this is the first report of a bacterium closely resembling P. mandelii infecting and causing disease in fish. The bacterium grew at temperatures between 5°C and 30°C, but not at 37°C. It is possible that infections in fish were a result of immunosuppression associated with spring conditions combined with the psychrotrophic nature of the bacterium.