Association between the Trp64Arg polymorphism of the ADRB3 gene and overactive bladder

• Published in: Neurourology and urodynamics Vol. 40; no. 7; pp. 1780 - 1785
• Main Authors: Çırakoğlu, Abdullah, Fejzullahu, Arta, Benli, Erdal, Yuce, Ahmet, Ayyıldız, Ali, Aynacıoğlu, A. Şükrü
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.09.2021
• Subjects: Adrenergic receptors; Alleles; Female; Gene polymorphism; Humans; Male; overactive bladder; Polymerase chain reaction; Polymorphism; Polymorphism, Genetic; Receptors, Adrenergic, beta-3 - genetics; Restriction fragment length polymorphism; Statistical analysis; Trp64Arg polymorphism; Urinary Bladder, Overactive - genetics; ß3‐adrenoceptor; Trp64Arg polymorphism; overactive bladder; ß3-adrenoceptor
• ISSN: 0733-2467; 1520-6777; 1520-6777
• DOI: 10.1002/nau.24742

Background The β3‐adrenergic receptor (ADRB3) is very important in the regulation of the human detrusor muscle function. The well‐known tryptophan64arginine polymorphism of the ADRB3 gene alters the response of the receptor to various stimuli, including adrenalin and noradrenalin, and may increase the susceptibility to develop overactive bladder (OAB). Therefore, this study was performed to determine whether ADRB3 Trp64Arg polymorphism is associated with the pathophysiology of OAB syndrome. Methods The study group (n = 150) consists of 72 patients with OAB and 78 controls without OAB. Venous blood samples were taken from all participants to analyze the ADRB3 gene Trp64Arg polymorphism using polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP) assay. We compared the distribution of Trp64Arg polymorphism and symptom severity in both OAB and non‐OAB subjects using χ2 test and Mann–Whitney's U test, respectively. Results The frequency of the 64Arg variant (heterozygous plus homozygous) in OAB and non‐OAB subjects was 15.3% and 14.1%, respectively. There was no statistically significant difference between the OAB and non‐OAB groups in regard to the distribution frequency of ADRB3 Trp64Arg polymorphism. The total frequency (OAB + non‐OAB, 76 women and 74 men) of the Arg64 variant allele was 5.9% and 10.8% in women and in men, respectively. Although the frequency of the Arg64 variant was nearly twofold higher in men compared to women, the difference was not statistically significant. Conclusions These results demonstrated that the ADRB3 Trp64Arg polymorphism is not significantly associated with OAB syndrome in a sample of Turkish OAB patients.