Repeatability of in vitro carboplatin elution from carboplatin‐impregnated calcium sulfate hemihydrate beads made in a clinic setting

• Published in: Veterinary surgery Vol. 49; no. 8; pp. 1609 - 1617
• Main Authors: Worth, David B., Risselada, Marije, Cooper, Bruce R., Moore, George E.
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.12.2020; Blackwell Publishing Ltd
• Subjects: Animals; Beads; Calcium; Calcium sulfate; Calcium Sulfate - chemistry; Calcium sulfate hemihydrate; Carboplatin; Carboplatin - chemistry; Cats; Coefficient of variation; Dilution; Dogs; Dosage; Elution; In vitro methods and tests; Learning curves; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Mathematical analysis; Microspheres; Performance evaluation; Setting (hardening); Sulfates; Variation
• ISSN: 0161-3499; 1532-950X
• DOI: 10.1111/vsu.13511

Objective To evaluate the intra‐lot and inter‐lot consistency and total carboplatin elution over 25 days from carboplatin‐impregnated calcium sulfate hemihydrate (C‐I CSH) beads manufactured in a clinic setting. Study design In vitro elution study. Methods Two volumes of carboplatin were mixed with CSH to yield 4 mg and 8 mg C‐I CSH doses. Two lots of beads were made for each concentration and split into five doses (n = 10 per concentration). Beads hardened in molds and were placed in a covered six‐well plate, submerged in phosphate‐buffered saline, and incubated with samples collected at 12 time points (0, 6, 12, and 24 hours and 2, 3, 5, 7, 10, 14, 18, and 25 days). The amount of carboplatin in each sample was evaluated by high‐performance liquid chromatography/mass spectrometry. Correction for carboplatin degradation and dilution was applied, and eluted carboplatin was calculated. Intra‐lot and inter‐lot coefficient of variation (CV) was calculated for each concentration. Results The intra‐lot CV ranged between 7.9% and 23.1%, and the inter‐lot CV ranged from 3.5% to 10.3%, with improvement noted in each successive lot of beads. Mean peak eluted carboplatin was 2.45 ± 0.43 mg (61%) and 3.68 ± 0.41 mg (45.9%) for the 4‐mg and 8‐mg C‐I CSH beads, respectively, with both occurring at the 12‐hour timepoint. Conclusion Progressive improvement in variability with successive lots of beads indicated a learning curve with bead manufacturing with a low variation both within and between lots of C‐I CSH beads. Clinical significance On‐site mixing of carboplatin with commercial CSH bead powder leads to a low variation of carboplatin per bead dose.