Berberine demonstrates anti‐inflammatory properties in Helicobacter pylori‐infected mice with chronic gastritis by attenuating the Th17 response triggered by the B cell‐activating factor

• Published in: Journal of cellular biochemistry Vol. 119; no. 7; pp. 5373 - 5381
• Main Authors: Wu, Xiuxia, Li, Xian, Dang, Zhongqin, Jia, Yunfei
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.07.2018
• Subjects: Anticancer properties; Antidiabetics; Antitumor activity; BAFF; Berberine; BLyS protein; CD28 antigen; CD3 antigen; CD4 antigen; chronic gastritis; Cytokines; Diabetes mellitus; Gastritis; Gene expression; H. pylori; Helicobacter pylori; Helper cells; Herbal medicine; IL-1β; Inflammation; Lymphocytes; Lymphocytes B; Lymphocytes T; Macrophages; Medicinal herbs; Medicinal plants; Mice; Mucosa; Mucous membrane; Th17; BAFF; H. pylori; Th17; berberine; chronic gastritis
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.26681

Berberine (BBR) is an isoquinoline alkaloid derived from various medicinal herbs. Previous studies have suggested that BBR exerts antimicrobial, antitumor, and antidiabetic effects and can be used to treat Helicobacter pylori‐induced chronic gastritis. However, the exact mechanism by which BBR inhibits H. pylori infection is not fully understood. We investigated the anti‐inflammatory properties and potential mechanism of BBR in H. pylori‐infected mice with chronic gastritis. We found that BBR can suppress the expression of pro‐inflammatory genes IL‐6, TGF‐β, and IL‐1β and upregulate anti‐inflammatory gene IL‐10 expression in the mucosa and RAW 264.7 macrophages. Exposure to BBR also reduced the expression and accumulation of IL‐17 in the mucosa and CD4+ T cells activated by anti‐CD3 and anti‐CD28, and it decreased the frequency of IL‐17‐producing CD4+ T cells. B cell‐activating factor (BAFF) production was inhibited by BBR and by cultured dendritic and CD4+ T cells. Furthermore, we demonstrated that BAFF can trigger the Th17 response by promoting the production of pro‐Th17 cytokines IL‐6, TGF‐β, and IL‐1β, which are strongly associated with the anti‐inflammatory role of BBR in chronic gastritis caused by H. pylori. In conclusion, we determined that BBR has anti‐inflammatory effects on H. pylori‐induced chronic gastritis by attenuating the BAFF‐triggered Th17 response. We determined that BBR has anti‐inflammatory effects on H. pylori‐induced chronic gastritis by attenuating the BAFF‐triggered Th17 response.