Development of a new diaper dermatitis‐like reconstructed skin equivalent for testing children atopic dermatitis relieving cosmetics

• Published in: Skin research and technology Vol. 25; no. 6; pp. 839 - 845
• Main Authors: Zhuang, Linyi, Gu, Hongjian, Huang, Yu, Li, Xiao, Lu, Yongbo, Kaku, Ken
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.11.2019
• Subjects: Anti-Inflammatory Agents - pharmacology; anti‐inflammation; Atopic dermatitis; Cells, Cultured; Children; Cosmetics; Cosmetics industry; Dermatitis; Dermatitis, Atopic; Diaper dermatitis; Diaper rash; Diaper Rash - pathology; Diaper Rash - physiopathology; Eczema; Equivalence; Humans; In vitro methods and tests; Inflammation; Interleukin-1alpha - antagonists & inhibitors; Interleukin-1alpha - metabolism; Interleukins; Irritation; Keratinocytes - drug effects; Models, Biological; Organic chemistry; Protective Agents - pharmacology; Quality of life; reconstructed human skin equivalent in vitro; Signs and symptoms; Skin; Skin - drug effects; Skin - physiopathology; skin barrier; Skin Cream - pharmacology; Skin diseases; Skin tests; Sodium; Sodium Dodecyl Sulfate; Sodium lauryl sulfate; Tissues; Viability; skin barrier; Diaper dermatitis; anti-inflammation; reconstructed human skin equivalent in vitro
• ISSN: 0909-752X; 1600-0846
• DOI: 10.1111/srt.12731

Background Diaper dermatitis (DD) is the most common acute inflammatory skin disease. It has a serious effect on children's and families’ quality of life. We aimed to screen and evaluate the efficacy of different formulas for relieving the diaper dermatitis symptoms by developing a kind of diaper dermatitis‐like reconstructed human skin equivalent in vitro. Materials and Method We developed the human skin equivalent for diaper dermatitis with 0.2% Sodium lauryl sulfate (SLS). The diaper dermatitis‐like human skin equivalent was characterized by high level of inflammation, such as overexpression of interleukin‐1α (IL‐1α), and impaired skin barrier. Four formulas with potential of anti‐inflammation and promotion of skin barrier function were topically applied on the diaper dermatitis‐like human skin equivalent surface. The afterward protection efficacy was evaluated by endpoints of IL‐1α, tissue viability, and skin barrier function. Results The chemical irritant induced high release of IL‐1α, impaired tissue viability, and skin barrier function. The cream prepared with potential of anti‐inflammation and skin protection could effectively decrease and relive the impact of irritant with decreased level of IL‐1α and the higher tissue viability than the placebo exposure. Conclusion The results showed that diaper dermatitis‐like human skin equivalent induced by SLS can mimic the skin irritation response of the diaper rash.