UGT89AC1‐mediated quercetin glucosylation is induced upon herbivore damage and enhances Camellia sinensis resistance to insect feeding

• Published in: Plant, cell and environment Vol. 47; no. 2; pp. 682 - 697
• Main Authors: Jing, Tingting, Du, Wenkai, Qian, Xiaona, Wang, Kai, Luo, Lanxin, Zhang, Xueying, Deng, Yanni, Li, Bo, Gao, Ting, Zhang, Mengting, Guo, Danyang, Jiang, Hao, Liu, Yuantao, Schwab, Wilfried, Sun, Xiaoling, Song, Chuankui
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.02.2024
• Subjects: Animals; Artificial diets; Camellia sinensis; Camellia sinensis - metabolism; CsUGT89AC1; Cytoplasm; Diet; Ecological effects; Flavone glycosides; Flavonoids; Flavonols; Glucosides; Glucosides - metabolism; Glycosides; herbivore resistance; Herbivory; Insects; Larva; Larvae; Larval development; Lepidoptera; Plant Proteins - metabolism; Quercetin; Quercetin - metabolism; Quercetin - pharmacology; quercetin glucoside; Tea; Tea - metabolism; Camellia sinensis; herbivore resistance; CsUGT89AC1; quercetin glucoside
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1111/pce.14751

Quercetin is a key flavonol in tea plants (Camellia sinensis (L.) O. Kuntze) with various health benefits, and it often occurs in the form of glucosides. The roles of quercetin and its glucosylated forms in plant defense are generally not well‐studied, and remain unknown in the defense of tea. Here, we found higher contents of quercetin glucosides and a decline of the aglucone upon Ectropis grisescens (E. grisescens) infestation of tea. Nine UGTs were strongly induced, among which UGT89AC1 exhibited the highest activity toward quercetin in vitro and in vivo. The mass of E. grisescens larvae that fed on plants with repressed UGT89AC1 or varieties with lower levels of UGT89AC1 was significantly lower than that of larvae fed on controls. Artificial diet supplemented with quercetin glucoside also reduced the larval growth rate, whereas artificial diet supplemented with free quercetin had no significant effect on larval growth. UGT89AC1 was located in both the cytoplasm and nucleus, and its expression was modulated by JA, JA‐ILE, and MeJA. These findings demonstrate that quercetin glucosylation serves a defensive role in tea against herbivory. Our results also provide novel insights into the ecological relevance of flavonoid glycosides under biotic stress in plants. Summary Statement Quercetin is a key flavonol in plants and often occurs in the form of glucosides, whereas the roles of glucosylated quercetin in plant defense are generally not well‐studied, especially in tea plants. We proved that quercetin glucosylation mediated by UGT89AC1 is induced upon herbivore damage and enhances plant resistance to insect feeding in tea plants. The findings of our study provide novel insights into the ecological roles of flavonoid glycosides under biotic stress in plants.