Hsa_circ_0008500 inhibits apoptosis of adipose‐derived stem cells under high glucose through hsa‐miR‐1273h‐5p/ELK1 axis

• Published in: Environmental toxicology Vol. 38; no. 7; pp. 1732 - 1742
• Main Authors: Meng, Fandong, Shen, Fengjie, Chu, Xuan, Ling, Hongwei, Qiao, Yun, Liu, Deshan
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.07.2023; Wiley Subscription Services, Inc
• Subjects: Adipose tissue; ADSCs; Apoptosis; Circular RNA; Diabetes mellitus; ELK1; Glucose; hsa_circ_0008500; hsa‐miR‐1273h‐5p; Mercury; miRNA; Signal transduction; Stem cells; Wound healing; ADSCs; ELK1; hsa-miR-1273h-5p; hsa_circ_0008500
• ISSN: 1520-4081; 1522-7278
• DOI: 10.1002/tox.23801

Preliminary researches have confirmed that the number of apoptosis of adipose tissue‐derived stem cells (ADSCs) in patients with diabetes is significantly increased, leading to a difficult healing wound. Increasing researches revealed that circular RNAs (circRNAs) can control apoptosis. However, it is still unclear whether and how circRNAs are critical for regulating ADSCs apoptosis. In this study, we utilized in vitro model in which ADSCs were cultivated with normal glucose (NG) (5.5 mM) or high glucose (HG) (25 mM) medium, respectively, and found that more apoptotic ADSCs were observed in HG medium comparing to ADSCs in NG medium. Furthermore, we found that hsa_circ_0008500 attenuated HG‐mediated ADSCs apoptosis. In addition, Hsa_circ_0008500 could directly interact with hsa‐miR‐1273h‐5p, acting as a miRNA sponge, which subsequently suppressed Ets‐like protein‐1(ELK1) expression, the downstream target of hsa‐miR‐1273h‐5p. Thus, these results indicated that targeting the hsa_circ_0008500/hsa‐miR‐1273h‐5p/ELK1 signaling pathway in ADSCs may be a potential target for repairing diabetic wounds.