Kangfuxin Accelerates Extraction Socket Healing by Promoting Angiogenesis Via Upregulation of CCL2 in Stem Cells

• Published in: Journal of bone and mineral research Vol. 38; no. 8; pp. 1208 - 1221
• Main Authors: Gao, Zheng‐Rong, Zhou, Ying‐Hui, Zhao, Ya‐Qiong, Zhao, Jie, Ye, Qin, Zhang, Shao‐Hui, Feng, Yao, Tan, Li, Liu, Qiong, Chen, Yun, Ouyang, Ze‐Yue, Hu, Jing, Dusenge, Marie Aimee, Feng, Yun‐Zhi, Guo, Yue
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.08.2023; Wiley Subscription Services, Inc
• Subjects: ANGIOGENESIS; Bone growth; Bone healing; Bone mass; CCL2; Cell migration; Chemokines; Dental pulp; Endothelial cells; EXTRACTION SOCKET HEALING; KANGFUXIN; Mesenchymal stem cells; Mineralization; Monocyte chemoattractant protein 1; Osteogenesis; Periodontal ligament; STEM CELLS; Wound healing; CCL2; STEM CELLS; ANGIOGENESIS; KANGFUXIN; EXTRACTION SOCKET HEALING
• ISSN: 0884-0431; 1523-4681
• DOI: 10.1002/jbmr.4860

ABSTRACT Kangfuxin (KFX) shows potential in wound healing, but its role in socket healing is unclear. This research finds increased bone mass, mineralization, and collagen deposition in KFX‐treated mice. Mouse bone marrow mesenchymal stem cells, human periodontal ligament stem cells (hPDLSCs), and human dental pulp stem cells (hDPSCs) are treated with KFX under osteogenic induction. RNA‐sequencing reveals upregulated chemokine‐related genes, with a threefold increase in chemokine (C‐C motif) ligand 2 (Ccl2). The conditioned medium (CM) of hPDLSCs and hDPSCs treated with KFX promotes endothelial cell migration and angiogenesis. Ccl2 knockdown abolishes CM‐induced endothelial cell migration and angiogenesis, which can be reversed by recombinant CCL2 treatment. KFX‐treated mice showed increased vasculature. In conclusion, KFX increases the expression of CCL2 in stem cells, promoting bone formation and mineralization in the extraction socket by inducing endothelial cell angiogenesis. © 2023 American Society for Bone and Mineral Research (ASBMR). Kangfuxin, a traditional Chinese medicine, increases the expression of chemokine (C‐C motif) ligand 2 (CCL2) protein in stem cells (bone marrow mesenchymal stem cells, periodontal ligament stem cells, and dental pulp stem cells). CCL2 secreted from stem cells induces endothelial cell angiogenesis, which promotes socket healing by enhancing bone formation and mineralization.