Ascorbic acid ameliorates dysregulated folliculogenesis induced by mono‐(2‐ethylhexyl)phthalate in neonatal mouse ovaries via reducing ovarian oxidative stress

Phthalates, including di‐(2‐ethylhexyl)phthalate (DEHP), are common industrial chemicals in the environment. Recent evidence indicates that DEHP and its active metabolite mono‐(2‐ethylhexyl)phthalate (MEHP) negatively modulate reproductive functions and induce reactive oxygen species. Ascorbic acid...

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Published inReproduction in domestic animals Vol. 55; no. 10; pp. 1418 - 1424
Main Authors Liu, Chang, Shui, Shike, Yao, Yangcheng, Sui, Cong, Zhang, Hanwang
Format Journal Article
LanguageEnglish
Published Germany Blackwell Publishing Ltd 01.10.2020
Subjects
Ascorbic acid
Constitution
Diet
Dioctyl phthalate
Follicles
Folliculogenesis
Free radicals
Malondialdehyde
Metabolites
mono‐(2‐ethylhexyl)phthalate
Neonates
ovarian follicle
Ovaries
ovary
Oxidative stress
Phthalates
Primates
Reactive oxygen species
Superoxide dismutase
Toxicity
ovarian follicle
ascorbic acid
ovary
oxidative stress
mono-(2-ethylhexyl)phthalate
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Summary:Phthalates, including di‐(2‐ethylhexyl)phthalate (DEHP), are common industrial chemicals in the environment. Recent evidence indicates that DEHP and its active metabolite mono‐(2‐ethylhexyl)phthalate (MEHP) negatively modulate reproductive functions and induce reactive oxygen species. Ascorbic acid (AA) is a dietary requirement for primates, and it acts as a potent free radical scavenger to protect tissues against oxidative stress. In this study, to investigate the toxic effects of MEHP on the follicle development and the beneficial role of AA, neonatal mouse ovaries were treated with different concentrations of MEHP with or without AA for 6 days. Then, the follicle constitution and oxidative status were compared in different groups. Results showed MEHP accelerated primordial follicle recruitment by increasing the percentage of primary and secondary follicles and decreasing the percentage of primordial follicles in the ovaries. Moreover, MEHP‐induced ovarian oxidative stress by significantly increasing malondialdehyde (MDA) concentration and the expression of GSS and SOD1. When ovaries were co‐administrated with MEHP and AA, follicle constitution was normalized, and the oxidative status was significantly decreased. These results suggested that AA ameliorated MEHP‐induced ovarian oxidative stress and follicular dysregulation, which attested the clinical significance of AA for ovary protection in the case of MEHP exposure.
Bibliography:Funding information
This work was supported by National Natural Science Foundation of China (grant number 81771582).
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13790