Stimulatory effects of platycodin D on osteoblast differentiation

Previous studies have suggested that platycodin D is implicated in bone biology and ameliorates osteoporosis development. Platycodin D repressed the osteoclast activity and enhanced bone mineral density in the mouse model. However, the effects of platycodin D on osteoblast differentiation have not b...

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Published inJournal of cellular biochemistry Vol. 120; no. 8; pp. 13085 - 13094
Main Authors Han, Younho, Jin, Sun Woo, Lee, Gi Ho, Choi, Jae Ho, Lee, Hyun Sun, Chung, Young Chul, Jeong, Hye Gwang, Lee, Kwang Youl
Format Journal Article
LanguageEnglish
Published United States Wiley Subscription Services, Inc 01.08.2019
Subjects
Alkaline phosphatase
Biocompatibility
Bone mineral density
Bone sialoprotein
Catenin
Cbfa-1 protein
Chemical compounds
Collagen (type I)
Differentiation
GSK3β
Mineralization
osteoblast
Osteoblastogenesis
Osteoporosis
Pharmacology
Phosphorylation
platycodin D
sirtuin 1 (SIRT1)
Transcription factors
β‐catenin
GSK3β
β-catenin
platycodin D
osteoblast
sirtuin 1 (SIRT1)
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Summary:Previous studies have suggested that platycodin D is implicated in bone biology and ameliorates osteoporosis development. Platycodin D repressed the osteoclast activity and enhanced bone mineral density in the mouse model. However, the effects of platycodin D on osteoblast differentiation have not been elucidated yet. In C3H10T1/2 cells, platycodin D upregulated osteogenic markers including alkaline phosphatase (ALP), bone sialoprotein, and collagen type 1 alpha 1, and transcription factors, such as Runx2 and osterix, subsequently enhancing the bone mineralization. In a molecular mechanism study, platycodin D induced β‐catenin nuclear accumulation by upregulating GSK3β phosphorylation. Furthermore, platycodin D upregulated the ALP activity and enhanced the mineralization process in osteoblast cells via the sirtuin 1/β‐catenin pathways. Taken together, these results suggested that platycodin D could be an effective therapeutic compound against osteoporosis because of its regulatory effects during the osteoblast differentiation. Platycodin D induces β‐catenin nuclear accumulation by inactivation of GSK3β. Platycodin D increases osteogenesis by enhancing the sirtuin 1 (SIRT1) activity.
Bibliography:Younho Han and Sun Woo Jin authors contributed equally to the work.
ObjectType-Article-1
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ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.28580