Expression of TWIST in the first‐trimester trophoblast and decidual tissue of women with recurrent pregnancy losses

• Published in: American journal of reproductive immunology (1989) Vol. 78; no. 2
• Main Authors: Ahn, Jin Hee, Park, Hye Ran, Park, Chan‐Woo, Park, Dong‐Wook, Kwak‐Kim, Joanne
• Format: Journal Article
• Language: English
• Published: Denmark Wiley Subscription Services, Inc 01.08.2017
• Subjects: Abortion; Abortion, Habitual - metabolism; Adult; Cell culture; Cell Nucleus - metabolism; Cells, Cultured; Cytoplasm; Cytoplasm - metabolism; Decidua; Decidua - metabolism; Endometrium; Female; first‐trimaster; Health risk assessment; Humans; Immunohistochemistry; Miscarriage; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Nuclei; Placenta; Polymerase chain reaction; Pregnancy; Pregnancy Trimester, First - metabolism; Stromal cells; trophoblast; Trophoblasts - metabolism; TWIST; Twist-Related Protein 1 - genetics; Twist-Related Protein 1 - metabolism; endometrium; trophoblast; decidua; first-trimaster; pregnancy; TWIST
• ISSN: 1046-7408; 1600-0897
• DOI: 10.1111/aji.12670

Problem In this study, we aim to investigate if the expression of TWIST in the first‐trimester placenta and decidua has any association with spontaneous abortion (SAB) and recurrent pregnancy losses (RPL). Methods Expression of TWIST was measured in the first‐trimester trophoblast and decidual tissues using RT‐PCR and immunohistochemistry. Women with a pregnancy loss comprised the study group (AB group) including women with two or more recurrent pregnancy loses (RPL group), and women with a spontaneous abortion who had no history of previous pregnancy losses (SAB group). Controls were women with elective abortion of normal pregnancy (NP group). Results In in‐vitro culture study of endometrial cells, expression of TWIST was detected with the induction of decidualization. In syncytiotrophoblast, expression of TWIST in NP group was significantly higher than that of AB group. RPL group had no expression of TWIST, both in the nucleus and cytoplasm of syncytiotrophoblast. In decidual tissue, majority of TWIST was expressed on the decidual stromal cells. NP group showed significantly higher expression of TWIST in cytoplasm and nucleus as compared with those of SAB group. Conclusion This suggests that TWIST may have a functional role in decidualization of endometrial cells and syncytialization of cytotrophoblast cells.