Effects of quorum‐sensing inhibition on experimental periodontitis induced by mixed infection in mice

• Published in: European journal of oral sciences Vol. 126; no. 6; pp. 449 - 457
• Main Authors: Ben Amara, Heithem, Song, Hyun Y., Ryu, Eunju, Park, Ji S., Schwarz, Frank, Kim, Byeong M., Choi, Bong‐Kyu, Koo, Ki‐Tae
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.12.2018
• Subjects: Alveolar bone; Alveolar Bone Loss; animal experimentation; Animals; antibacterial agents; Bacteria; Bacterial infections; Bone loss; Coinfection; Colonization; Computed tomography; Dentistry; Deoxyribonucleic acid; Detection; Disease Models, Animal; DNA; DNA, Bacterial - analysis; Furans - administration & dosage; Furans - antagonists & inhibitors; Fusobacterium nucleatum - genetics; Fusobacterium nucleatum - pathogenicity; Gene Expression; Genes, Bacterial; Gum disease; Host-Pathogen Interactions; host–parasite interactions; In vivo methods and tests; Infections; Inhibition; Inhibitors; Inoculation; Male; Mice; Mice, Inbred BALB C; Mixed infection; Periodontitis; Periodontitis - diagnostic imaging; Periodontitis - microbiology; Periodontitis - pathology; Porphyromonas gingivalis - genetics; Porphyromonas gingivalis - pathogenicity; Quorum Sensing - drug effects; Ribose; Ribose - administration & dosage; Ribose - antagonists & inhibitors; Tissues; X-Ray Microtomography; animal experimentation; host-parasite interactions; antibacterial agents; periodontitis
• ISSN: 0909-8836; 1600-0722
• DOI: 10.1111/eos.12570

This study aimed to verify, in in vivo settings, whether quorum‐sensing inhibition molecules could attenuate alveolar bone loss induced by Porphyromonas gingivalis/Fusobacterium nucleatum co‐infection and reduce the bacterial colonization of periodontal tissues. In BALB/c mice, periodontitis was induced through oral inoculation with P. gingivalis and F. nucleatum six times during a 42‐d period. Quorum sensing inhibitors (a furanone compound and D‐ribose) were administered simultaneously with bacterial infection. Linear and volumetric modifications of interproximal alveolar bone levels were compared between groups using micro‐computed tomography. Total bacteria, and P. gingivalis and F. nucleatum DNA in periodontal tissues, were quantified using real‐time PCR. Radiographic linear measurements demonstrated a significant reduction of alveolar bone loss, of approximately 40%, in mice treated with quorum sensing inhibitors when compared with the co‐infection group. This was confirmed by a significant increase of residual bone volume in the test group. While total bacterial genes in the treatment group significantly decreased by 93% in periodontal tissue samples when quorum sensing inhibitors were administered, no significant differences of P. gingivalis DNA were found. Quorum sensing inhibitors reduced periodontal breakdown and bacterial infection in periodontal tissues after co‐infection with P. gingivalis and F. nucleatum.