Long noncoding RNA LINC00339 promotes laryngeal squamous cell carcinoma cell proliferation and invasion via sponging miR‐145

• Published in: Journal of cellular biochemistry Vol. 120; no. 5; pp. 8272 - 8279
• Main Authors: Liu, Shouzhou, Duan, Wenchao
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.05.2019
• Subjects: Apoptosis; Cell proliferation; Infiltration; Laryngeal cancer; laryngeal squamous cell carcinoma; LINC00339; long noncoding RNAs; Mesenchyme; miR‐145; Neck; Neoplasia; Ribonucleic acid; RNA; Squamous cell carcinoma; Tissues; long noncoding RNAs; LINC00339; laryngeal squamous cell carcinoma; miR-145
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.28110

Laryngeal squamous cell carcinoma (LSCC) is a very common neoplasm of the head and neck in the world. Long noncoding RNAs play key roles in cell infiltration, fate, apoptosis, and invasion. However, the functional role and expression of LINC00339 remains unclear in LSCC. In this study, we showed that the expression level of LINC00339 was upregulated in LSCC tissues and cell lines. LINC00339 silencing suppressed the proliferation, invasion, and epithelial‐mesenchymal transition (EMT) progression of LSCC cells. In addition, we showed that LINC00339 acted as a sponge of miR‐145, and LINC00339 silencing promoted the expression of miR‐145 in Hep2 cell. Furthermore, the expression of miR‐145 was lower in LSCC tissues than in their paired normal samples and the miR‐145 expression level was negatively correlated with LINC00339 expression in LSCC tissues. The knockdown of miR‐145 promoted the proliferation, invasion, and EMT progression of LSCC cells. Finally, we indicated that LINC00339 silencing inhibited the proliferation, invasion, and EMT progression of LSCC cells by suppressing the miR‐145 expression. These data suggested that LINC00339 acted as an oncogene in the development of LSCC, partly by regulating the miR‐145 expression. 1. The expression level of LINC00339 was upregulated in the laryngeal squamous cell carcinoma (LSCC) tissues and cell lines. 2. LINC00339 silencing suppressed the proliferation, invasion, and EMT progression of LSCC cells. 3. LINC00339 silencing inhibited the proliferation, invasion, and EMT progression of LSCC cells by suppressing miR‐145 expression.