Intersectin‐Cdc42 interaction is required for orderly meiosis in porcine oocytes

• Published in: Journal of cellular physiology Vol. 234; no. 5; pp. 7492 - 7497
• Main Authors: Li, Xiaoyan, Gao, Min, He, Yongfu, Xiong, Bo, Liu, Honglin, Gu, Ling
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.05.2019
• Subjects: Actin; Actins - metabolism; Adaptor proteins; Adaptor Proteins, Vesicular Transport - metabolism; Animals; Benzamides - pharmacology; Cdc42; cdc42 GTP-Binding Protein - metabolism; Cdc42 protein; Chromosome Segregation - drug effects; Chromosome Segregation - physiology; Chromosomes - drug effects; Chromosomes - metabolism; Female; Intersectin; intersectin (ITSN); Maturation; Meiosis; Meiosis - drug effects; Meiosis - physiology; oocyte; Oocytes; Oocytes - drug effects; Oocytes - metabolism; Polymerization; Proteins; reproduction; Spindle Apparatus - drug effects; Spindle Apparatus - metabolism; Spindle Apparatus - physiology; Swine; Thiourea - analogs & derivatives; Thiourea - pharmacology; meiosis; oocyte; intersectin (ITSN); Cdc42; reproduction
• ISSN: 0021-9541; 1097-4652
• DOI: 10.1002/jcp.27510

Intersectins (ITSNs) have been shown to act as adaptor proteins that govern multiple cellular events via regulating Cdc42 activity. However, it remains to be determined whether the ITSN‐Cdc42 pathway is functional in porcine oocytes. To address this question, we used a small molecule, ZCL278, to selectively disrupt the ITSN2‐Cdc42 interaction. In the present study, we find that porcine oocytes exposed to ZCL278 are unable to completely progress through meiosis. Meanwhile, the spindle defects and chromosomal congression failure are frequently detected in these oocytes. In support of this, we observed the accumulated distribution of vesicle‐like ITSN2 signals around the chromosome/spindle region during porcine oocyte maturation. In addition, our results also showed that inhibition of the ITSN‐Cdc42 interaction impairs the actin polymerization in porcine oocytes. In summary, the findings support a model where ITSNs, through the interaction with Cdc42, modulates the assembly of meiotic apparatus and actin polymerization, consequently ensuring the orderly meiotic progression during porcine oocyte maturation. Our findings support a model where intersectins, through the interaction with Cdc42, modulates the assembly of meiotic apparatus and actin polymerization, consequently ensuring the orderly meiotic progression during porcine oocyte maturation.