Interaction of Co, Mn, Mg and Al with d(GCGTACGC): a spectroscopic study

Spectroscopic study on the interactions of trace elements Co., Mn, Mg and Al with d(GCGTACGC) indicated the following: Al and Mg did not alter Tm values. Mn enhanced Tm at lower concentration and decreased it at higher concentrations. Interestingly Co at higher concentration elevated the Tm. These s...

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Published inMolecular biology reports Vol. 22; no. 1; p. 47
Main Authors Rajan, M T, Kumar, D C, Vishnuvardhan, D, Rao, K S, Viswamitra, M A
Format Journal Article
LanguageEnglish
Published Netherlands 1995
Subjects
Aluminum - pharmacology
Base Sequence
Binding Sites
Circular Dichroism
Cobalt - pharmacology
DNA - chemistry
DNA - drug effects
In Vitro Techniques
Kinetics
Magnesium - pharmacology
Manganese - pharmacology
Metals - pharmacology
Nucleic Acid Conformation - drug effects
Oligodeoxyribonucleotides - chemistry
Spectrometry, Fluorescence
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Summary:Spectroscopic study on the interactions of trace elements Co., Mn, Mg and Al with d(GCGTACGC) indicated the following: Al and Mg did not alter Tm values. Mn enhanced Tm at lower concentration and decreased it at higher concentrations. Interestingly Co at higher concentration elevated the Tm. These studies also showed lower concentrations of Mn displaced EtBr, whereas Al could displace it at higher ionic strength. Mg and Co displaced EtBr fluorescence at moderate concentrations. The binding constant values and CD spectra clearly indicated strong binding of these elements to DNA.
ISSN:0301-4851
DOI:10.1007/BF00996304