Preclinical PET Studies of [11C]UCB-J Binding in Minipig Brain
Purpose Loss of neuronal synapse function is associated with a number of brain disorders. The [ 11 C]UCB-J positron emission tomography (PET) tracer allows for in vivo examination of synaptic density, as it binds to synaptic vesicle glycoprotein 2A (SV2A) expressed in presynaptic terminals. Here, we...
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Published in | Molecular imaging and biology Vol. 22; no. 5; pp. 1290 - 1300 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Cham
Springer International Publishing
01.10.2020
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Purpose
Loss of neuronal synapse function is associated with a number of brain disorders. The [
11
C]UCB-J positron emission tomography (PET) tracer allows for
in vivo
examination of synaptic density, as it binds to synaptic vesicle glycoprotein 2A (SV2A) expressed in presynaptic terminals. Here, we characterise [
11
C]UCB-J imaging in Göttingen minipigs.
Procedures
Using PET imaging, we examined tracer specificity and compared kinetic models. We explored the use of a standard blood curve and centrum semiovale white matter as a reference region. We compared
in vivo
[
11
C]UCB-J PET imaging to
in vitro
autoradiography, Western blotting and real-time quantitative polymerase chain reaction.
Results
The uptake kinetics of [
11
C]UCB-J could be described using a 1-tissue compartment model and blocking of SV2A availability with levetiracetam showed dose-dependent specific binding. Population-based blood curves resulted in reliable [
11
C]UCB-J binding estimates, while it was not possible to use centrum semiovale white matter as a non-specific reference region. Brain [
11
C]UCB-J PET signals correlated well with [
3
H]UCB-J autoradiography and SV2A protein levels.
Conclusions
[
11
C]UCB-J PET is a valid
in vivo
marker of synaptic density in the minipig brain, with binding values close to those reported for humans. Minipig models of disease could be valuable for investigating the efficacy of putative neuroprotective agents for preserving synaptic function in future non-invasive, longitudinal studies. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1536-1632 1860-2002 |
DOI: | 10.1007/s11307-020-01506-8 |